阿尔及利亚珍珠粟（黍稷）含有木聚糖酶抑制蛋白（XIP），但不含有木聚糖酶抑制子（TAXI）和木聚糖酶抑制子样蛋白（TLXI）类型的木聚糖酶抑制剂。

Algerian pearl millet ( Pennisetum glaucum L.) contains XIP but not TAXI and TLXI type xylanase inhibitors.

作者信息

Mokrane Hind, Gebruers Kurt, Beaugrand Johnny, Proost Paul, Nadjemi Boubekeur, Belhanèche-Bensemra Naima, Courtin Christophe M, Delcour Jan A

机构信息

Laboratoire des Produits Bioactifs et de la Valorisation de la Biomasse, Ecole Normale Superieure, Vieux-Kouba, Alger, Algeria.

出版信息

J Agric Food Chem. 2009 Jun 24;57(12):5542-8. doi: 10.1021/jf9003785.

DOI:10.1021/jf9003785

PMID:19459708

Abstract

An XIP (xylanase inhibiting protein) type xylanase inhibitor was purified from Algerian pearl millet ( Pennisetum glaucum L.) grains and characterized for the first time. Cation exchange and affinity chromatography with immobilized Trichoderma longibrachiatum glycoside hydrolase (GH) family 11 xylanase resulted in electrophoretically pure protein with a molecular mass of 27-29 kDa and a pI value of 6.7. The experimentally determined N-terminal amino acid sequence of the purified XIP protein is 87.5%, identical to that of sorghum ( Sorghum bicolor L.) XIP and 79.2% identical to that of wheat ( Triticum aestivum L.) XIP-I. The biochemical properties of pearl millet XIP are comparable to those described earlier for sorghum XIP, except for the higher specific activity toward a T. longibrachiatum GH family 11 xylanase. On the basis of immunoblot neither TAXI nor TLXI type xylanase inhibitors were detected in pearl millet grains.

摘要

从阿尔及利亚珍珠粟（黍稷）种子中首次纯化并鉴定出一种XIP（木聚糖酶抑制蛋白）类型的木聚糖酶抑制剂。利用固定化长枝木霉糖苷水解酶（GH）家族11木聚糖酶进行阳离子交换和亲和层析，得到了电泳纯的蛋白质，其分子量为27 - 29 kDa，pI值为6.7。纯化后的XIP蛋白经实验测定的N端氨基酸序列与高粱（双色高粱）XIP的序列一致性为87.5%，与小麦（普通小麦）XIP - I的序列一致性为79.2%。珍珠粟XIP的生化特性与之前描述的高粱XIP的特性相当，只是对长枝木霉GH家族11木聚糖酶的比活性更高。基于免疫印迹法，在珍珠粟种子中未检测到TAXI和TLXI类型的木聚糖酶抑制剂。