Naldoni F J, Claudino A L R, Cruz J W, Chavasco J K, Faria e Silva P M, Veloso M P, Dos Santos M H
Departamento de Farmácia, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
J Med Food. 2009 Apr;12(2):403-7. doi: 10.1089/jmf.2007.0622.
The pericarp and seeds from fruits of Garcinia brasiliensis were subjected to extraction with hexane and ethanol. The pericarp hexane extract (PHE) and seed ethanol extract (SEE) were purified by silica gel column chromatography, which permitted isolation of the prenylated benzophenones 7-epiclusianone (1) and guttiferone-A (2), respectively. The antimicrobial activity of PHE, SEE, and compounds 1 and 2 were evaluated against Candida albicans, Staphylococcus aureus, Escherichia coli, and Bacillus cereus cultures. The minimum inhibitory concentration and minimum bactericidal concentration were established. The substances presented activity against S. aureus and B. cereus as follows: PHE, 4.0 microg/mL and 2.4 microg/mL; SEE, 10.0 microg/mL and 12.6 microg/mL; 7-epiclusianone, 1.2 microg/mL and 0.6 microg/mL; and guttiferone-A, 2.4 microg/mL and 2.4 microg/mL, respectively. The direct relationship between the lipophilic character of the structure and activity in Gram-positive bacteria was specifically observed. Therefore these extracts and prenylated benzophenones represent an interesting topic for further studies and open possibilities for an alternative control of diseases associated with Gram-positive bacteria.
对巴西藤黄果实的果皮和种子进行了己烷和乙醇提取。果皮己烷提取物（PHE）和种子乙醇提取物（SEE）通过硅胶柱色谱法进行纯化，分别得到了异戊烯基化二苯甲酮7-表clusianone（1）和藤黄酮-A（2）。评估了PHE、SEE以及化合物1和2对白色念珠菌、金黄色葡萄球菌、大肠杆菌和蜡样芽孢杆菌培养物的抗菌活性。确定了最低抑菌浓度和最低杀菌浓度。这些物质对金黄色葡萄球菌和蜡样芽孢杆菌的活性如下：PHE分别为4.0微克/毫升和2.4微克/毫升；SEE分别为10.0微克/毫升和12.6微克/毫升；7-表clusianone分别为1.2微克/毫升和0.6微克/毫升；藤黄酮-A分别为2.4微克/毫升和2.4微克/毫升。特别观察到结构的亲脂性与对革兰氏阳性菌的活性之间存在直接关系。因此，这些提取物和异戊烯基化二苯甲酮是进一步研究的有趣课题，并为与革兰氏阳性菌相关疾病的替代控制开辟了可能性。
