• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Synergistic effects of inward rectifier (I) and pacemaker (I) currents on the induction of bioengineered cardiac automaticity.内向整流（I）电流和起搏（I）电流对生物工程心脏自动性诱导的协同作用。
J Cardiovasc Electrophysiol. 2009 Sep;20(9):1048-54. doi: 10.1111/j.1540-8167.2009.01475.x.
2
Mechanistic role of I(f) revealed by induction of ventricular automaticity by somatic gene transfer of gating-engineered pacemaker (HCN) channels.通过门控工程化起搏器（HCN）通道的体细胞基因转移诱导心室自律性所揭示的I(f)的机制作用。
Circulation. 2007 Apr 10;115(14):1839-50. doi: 10.1161/CIRCULATIONAHA.106.659391. Epub 2007 Mar 26.
3
Overexpression of HCN-encoded pacemaker current silences bioartificial pacemakers.HCN编码的起搏电流过表达使生物人工起搏器失活。
Heart Rhythm. 2008 Sep;5(9):1310-7. doi: 10.1016/j.hrthm.2008.05.010. Epub 2008 May 15.
4
Automaticity and conduction properties of bio-artificial pacemakers assessed in an in vitro monolayer model of neonatal rat ventricular myocytes.在体外单层培养新生大鼠心室肌细胞模型中评估生物人工起搏器的自动性和传导特性。
Europace. 2010 Aug;12(8):1178-87. doi: 10.1093/europace/euq120. Epub 2010 May 14.
5
Bioartificial sinus node constructed via in vivo gene transfer of an engineered pacemaker HCN Channel reduces the dependence on electronic pacemaker in a sick-sinus syndrome model.通过工程化起搏器HCN通道的体内基因转移构建的生物人工窦房结可降低病态窦房结综合征模型对电子起搏器的依赖。
Circulation. 2006 Sep 5;114(10):1000-11. doi: 10.1161/CIRCULATIONAHA.106.615385. Epub 2006 Aug 21.
6
Recreating an artificial biological pacemaker: insights from a theoretical model.重建人工生物起搏器：来自理论模型的见解
Heart Rhythm. 2006 Jul;3(7):824-31. doi: 10.1016/j.hrthm.2006.03.012. Epub 2006 Mar 16.
7
Non-equilibrium behavior of HCN channels: insights into the role of HCN channels in native and engineered pacemakers.HCN通道的非平衡行为：对HCN通道在天然和工程化起搏器中作用的见解。
Cardiovasc Res. 2005 Aug 1;67(2):263-73. doi: 10.1016/j.cardiores.2005.03.006. Epub 2005 Apr 21.
8
Different intracellular polyamine concentrations underlie the difference in the inward rectifier K(+) currents in atria and ventricles of the guinea-pig heart.豚鼠心脏心房和心室内向整流钾电流的差异源于细胞内多胺浓度的不同。
J Physiol. 2005 Mar 15;563(Pt 3):713-24. doi: 10.1113/jphysiol.2004.077677. Epub 2005 Jan 24.
9
A Singular Role of I Promoting the Development of Cardiac Automaticity during Cardiomyocyte Differentiation by I -Induced Activation of Pacemaker Current.I 诱导起搏电流激活促进心肌细胞分化过程中心脏自动性的独特作用。
Stem Cell Rev Rep. 2017 Oct;13(5):631-643. doi: 10.1007/s12015-017-9745-1.
10
Kir2.1 channels set two levels of resting membrane potential with inward rectification.Kir2.1 通道通过内向整流设定静息膜电位的两个水平。
Pflugers Arch. 2018 Apr;470(4):599-611. doi: 10.1007/s00424-017-2099-3. Epub 2017 Dec 27.

引用本文的文献

1
Triiodothyronine and dexamethasone alter potassium channel expression and promote electrophysiological maturation of human-induced pluripotent stem cell-derived cardiomyocytes.三碘甲状腺原氨酸和地塞米松改变钾通道表达并促进人诱导多能干细胞衍生心肌细胞的电生理成熟。
J Mol Cell Cardiol. 2021 Dec;161:130-138. doi: 10.1016/j.yjmcc.2021.08.005. Epub 2021 Aug 13.
2
Mitochondria and metabolic transitions in cardiomyocytes: lessons from development for stem cell-derived cardiomyocytes.心肌细胞中的线粒体和代谢转换：来自干细胞衍生的心肌细胞发育的启示。
Stem Cell Res Ther. 2021 Mar 12;12(1):177. doi: 10.1186/s13287-021-02252-6.
3
Sinus node-like pacemaker mechanisms regulate ectopic pacemaker activity in the adult rat atrioventricular ring.窦房结样起博机制调节成年大鼠房室环异位起博活性。
Sci Rep. 2019 Aug 13;9(1):11781. doi: 10.1038/s41598-019-48276-0.
4
Gene Therapy Approaches to Biological Pacemakers.生物起搏器的基因治疗方法。
J Cardiovasc Dev Dis. 2018 Oct 19;5(4):50. doi: 10.3390/jcdd5040050.
5
Kir2 inward rectification-controlled precise and dynamic balances between Kir2 and HCN currents initiate pacemaking activity.内向整流型钾通道 2（Kir2）的内向整流控制作用精确而动态地平衡 Kir2 和 HCN 电流，从而引发起搏活动。
FASEB J. 2018 Jun;32(6):3047-3057. doi: 10.1096/fj.201701260R. Epub 2018 Jan 12.
6
A Singular Role of I Promoting the Development of Cardiac Automaticity during Cardiomyocyte Differentiation by I -Induced Activation of Pacemaker Current.I 诱导起搏电流激活促进心肌细胞分化过程中心脏自动性的独特作用。
Stem Cell Rev Rep. 2017 Oct;13(5):631-643. doi: 10.1007/s12015-017-9745-1.
7
Tachycardia-bradycardia syndrome: Electrophysiological mechanisms and future therapeutic approaches (Review).心动过速-心动过缓综合征：电生理机制及未来治疗方法（综述）
Int J Mol Med. 2017 Mar;39(3):519-526. doi: 10.3892/ijmm.2017.2877. Epub 2017 Feb 6.
8
Enhancement of Spontaneous Activity by HCN4 Overexpression in Mouse Embryonic Stem Cell-Derived Cardiomyocytes - A Possible Biological Pacemaker.通过在小鼠胚胎干细胞衍生的心肌细胞中过表达HCN4增强自发活动——一种可能的生物起搏器
PLoS One. 2015 Sep 18;10(9):e0138193. doi: 10.1371/journal.pone.0138193. eCollection 2015.
9
Stem cell-based biological pacemakers from proof of principle to therapy: a review.基于干细胞的生物起搏器：从原理验证到治疗的综述
Cytotherapy. 2014 Jul;16(7):873-80. doi: 10.1016/j.jcyt.2014.02.014. Epub 2014 May 13.
10
Electrical stimulation promotes maturation of cardiomyocytes derived from human embryonic stem cells.电刺激促进人胚胎干细胞来源的心肌细胞成熟。
J Cardiovasc Transl Res. 2013 Dec;6(6):989-99. doi: 10.1007/s12265-013-9510-z. Epub 2013 Oct 1.

本文引用的文献

1
Overexpression of HCN-encoded pacemaker current silences bioartificial pacemakers.HCN编码的起搏电流过表达使生物人工起搏器失活。
Heart Rhythm. 2008 Sep;5(9):1310-7. doi: 10.1016/j.hrthm.2008.05.010. Epub 2008 May 15.
2
Structural and functional determinants in the S5-P region of HCN-encoded pacemaker channels revealed by cysteine-scanning substitutions.通过半胱氨酸扫描替换揭示的HCN编码的起搏器通道S5-P区域的结构和功能决定因素。
Am J Physiol Cell Physiol. 2008 Jan;294(1):C136-44. doi: 10.1152/ajpcell.00340.2007. Epub 2007 Nov 7.
3
HCN-encoded pacemaker channels: from physiology and biophysics to bioengineering.HCN编码的起搏器通道：从生理与生物物理学到生物工程学
J Membr Biol. 2006;214(3):115-22. doi: 10.1007/s00232-006-0881-9. Epub 2007 Jun 8.
4
New insights into pacemaker activity: promoting understanding of sick sinus syndrome.起搏器活动的新见解：促进对病态窦房结综合征的理解。
Circulation. 2007 Apr 10;115(14):1921-32. doi: 10.1161/CIRCULATIONAHA.106.616011.
5
Mechanistic role of I(f) revealed by induction of ventricular automaticity by somatic gene transfer of gating-engineered pacemaker (HCN) channels.通过门控工程化起搏器（HCN）通道的体细胞基因转移诱导心室自律性所揭示的I(f)的机制作用。
Circulation. 2007 Apr 10;115(14):1839-50. doi: 10.1161/CIRCULATIONAHA.106.659391. Epub 2007 Mar 26.
6
Bioartificial sinus node constructed via in vivo gene transfer of an engineered pacemaker HCN Channel reduces the dependence on electronic pacemaker in a sick-sinus syndrome model.通过工程化起搏器HCN通道的体内基因转移构建的生物人工窦房结可降低病态窦房结综合征模型对电子起搏器的依赖。
Circulation. 2006 Sep 5;114(10):1000-11. doi: 10.1161/CIRCULATIONAHA.106.615385. Epub 2006 Aug 21.
7
Wild-type and mutant HCN channels in a tandem biological-electronic cardiac pacemaker.串联式生物电子心脏起搏器中的野生型和突变型HCN通道
Circulation. 2006 Sep 5;114(10):992-9. doi: 10.1161/CIRCULATIONAHA.106.617613. Epub 2006 Aug 21.
8
Familial sinus bradycardia associated with a mutation in the cardiac pacemaker channel.与心脏起搏器通道突变相关的家族性窦性心动过缓。
N Engl J Med. 2006 Jan 12;354(2):151-7. doi: 10.1056/NEJMoa052475.
9
Non-equilibrium behavior of HCN channels: insights into the role of HCN channels in native and engineered pacemakers.HCN通道的非平衡行为：对HCN通道在天然和工程化起搏器中作用的见解。
Cardiovasc Res. 2005 Aug 1;67(2):263-73. doi: 10.1016/j.cardiores.2005.03.006. Epub 2005 Apr 21.
10
The inward rectifier current (IK1) controls cardiac excitability and is involved in arrhythmogenesis.内向整流电流（IK1）控制心脏兴奋性并参与心律失常的发生。
Heart Rhythm. 2005 Mar;2(3):316-24. doi: 10.1016/j.hrthm.2004.11.012.

内向整流（I）电流和起搏（I）电流对生物工程心脏自动性诱导的协同作用。

Synergistic effects of inward rectifier (I) and pacemaker (I) currents on the induction of bioengineered cardiac automaticity.

作者信息

Chan Yau-Chi, Siu Chung-Wah, Lau Yee-Man, Lau Chu-Pak, Li Ronald A, Tse Hung-Fat

机构信息

Cardiology Division, Department of Medicine, University of Hong Kong, Hong Kong.

出版信息

J Cardiovasc Electrophysiol. 2009 Sep;20(9):1048-54. doi: 10.1111/j.1540-8167.2009.01475.x.

DOI:10.1111/j.1540-8167.2009.01475.x
PMID:19460073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2739246/
Abstract

INTRODUCTION

Normal heart rhythms originate in the sinoatrial node. HCN-encoded funny current (I(f)) and the Kir2-encoded inward rectifier (I(K1)) counteract each other by respectively oscillating and stabilizing the negative resting membrane potential, and controlling action potential firing. Therefore, I(K1) suppression and I(f) overexpression have been independently exploited to convert cardiomyocytes (CMs) into AP-firing bioartificial pacemakers. Although the 2 strategies have been largely assumed synergistic, their complementarity has not been investigated.

METHODS AND RESULTS

We explored the interrelationships of automaticity, I(f) and I(K1) by transducing single left ventricular (LV) CMs isolated from guinea pig hearts with the recombinant adenoviruses Ad-CMV-GFP-IRES-HCN1-AAA and/or Ad-CGI-Kir2.1 to mediate their current densities via a whole-cell patch clamp technique at 37 degrees C. Results showed that Ad-CGI-HCN1-AAA but not Ad-CGI-Kir2.1 transduction induced automaticity (181.1 +/- 13.1 bpm). Interestingly, Ad-CGI-HCN1-AAA/Ad-CGI-Kir2.1 cotransduction significantly promoted the induced firing frequency (320.0 +/- 15.8 bpm; P < 0.05). Correlation analysis revealed that the firing frequency, phase-4 slope and APD(90) of AP-firing LV CMs were correlated with I(f) (R(2) > 0.7) only when -2 >I(K1) >-4 pA/pF but not with I(K1) over the entire I(f) ranges examined (0.02 < R(2) < 0.4). Unlike I(f), I(K1) displayed correlation with neither the phase-4 slope (R(2)= 0.02) nor phase-4 length (R(2)= 0.04) when -2 > I(f) > -4 pA/pF. As anticipated, however, APD(90) was correlated with I(K1) (R(2)= 0.4).

CONCLUSION

We conclude that an optimal level of I(K1) maintains a voltage range for I(f) to operate most effectively during a dynamic cardiac cycle.

摘要

引言

正常心脏节律起源于窦房结。由HCN编码的超极化激活的阳离子电流（I(f)）和由Kir2编码的内向整流钾电流（I(K1)）通过分别使静息膜电位振荡和稳定以及控制动作电位发放来相互拮抗。因此，I(K1)抑制和I(f)过表达已被分别用于将心肌细胞（CMs）转化为能发放动作电位的生物人工起搏器。尽管这两种策略在很大程度上被认为具有协同作用，但它们的互补性尚未得到研究。

方法与结果

我们通过用重组腺病毒Ad-CMV-GFP-IRES-HCN1-AAA和/或Ad-CGI-Kir2.1转导从豚鼠心脏分离的单个左心室（LV）心肌细胞，在37℃下通过全细胞膜片钳技术调节其电流密度，从而探究自律性、I(f)和I(K1)之间的相互关系。结果显示，Ad-CGI-HCN1-AAA转导而非Ad-CGI-Kir2.1转导诱导了自律性（181.1±13.1次/分钟）。有趣的是，Ad-CGI-HCN1-AAA/Ad-CGI-Kir2.1共转导显著提高了诱导的发放频率（320.0±15.8次/分钟；P<0.05）。相关性分析表明，仅当-2>I(K1)>-4 pA/pF时，发放动作电位的LV心肌细胞的发放频率、4期斜率和动作电位时程（APD(90)）与I(f)相关（R(2)>0.7），而在整个检测的I(f)范围内（0.02<R(2)<0.4）与I(K1)无关。与I(f)不同，当-2>I(f)>-4 pA/pF时，I(K1)与4期斜率（R(2)=0.02）和4期长度（R(2)=0.04）均无相关性。然而，正如预期的那样，APD(90)与I(K1)相关（R(2)=0.4）。

结论

我们得出结论，在动态心动周期中，I(K1)的最佳水平维持了I(f)最有效发挥作用的电压范围。