Hansen Carsten G, Nichols Benjamin J
MRC-LMB, Hills Road, Cambridge, CB2 0QH, UK.
J Cell Sci. 2009 Jun 1;122(Pt 11):1713-21. doi: 10.1242/jcs.033951.
There is good evidence that, in addition to the canonical clathrin-associated endocytic machinery, mammalian cells possess multiple sets of proteins that are capable of mediating the formation of endocytic vesicles. The identity, mechanistic properties and function of these clathrin-independent endocytic pathways are currently under investigation. This Commentary briefly recounts how the field of clathrin-independent endocytosis has developed to date. It then highlights recent progress in identifying key proteins that might define alternative types of endocytosis. These proteins include CtBP (also known as BARS), flotillins (also known as reggies) and GRAF1. We argue that a combination of information about pathway-specific proteins and the ultrastructure of endocytic invaginations provides a means of beginning to classify endocytic pathways.
有充分证据表明，除了典型的网格蛋白相关内吞机制外，哺乳动物细胞还拥有多组能够介导内吞小泡形成的蛋白质。目前正在研究这些非网格蛋白依赖性内吞途径的特性、机制属性和功能。本述评简要叙述了非网格蛋白依赖性内吞作用领域迄今为止的发展历程。然后重点介绍了在鉴定可能定义其他内吞类型的关键蛋白方面取得的最新进展。这些蛋白包括CtBP（也称为BARS）、flotillins（也称为reggies）和GRAF1。我们认为，关于途径特异性蛋白的信息与内吞凹陷超微结构的结合提供了一种开始对内吞途径进行分类的方法。
