Barth J, Petermann W, Zemke F, Kreipe H, Gross W L
Abteilung Allgemeine Innere Medizin, I. Medizinische Universitätsklinik Kiel.
Pneumologie. 1991 Jul;45(7):570-4.
Bronchoalveolar lavage was performed in 14 patients suffering from histologically confirmed Wegener's granulomatosis who also showed x-ray signs of lung involvement. Cell distribution and immunophenotypical characterisation of lymphocytes and alveolar macrophages of patients suffering from Wegener's granulomatosis were compared with the findings obtained from 10 controls. The bronchoalveolar lavage of the granulomatosis patients showed a significant increase of the total cell count, signalling inflammatory involvement of the lung. Differential cytgological analysis showed a significant increase in the total number of granulocytes in the lavage fluid of the Wegener's granulomatosis patients (in one case up to 40%). The median granulocyte count was increased fivefold compared with the control. The increase of the granulocyte count was mainly conditioned by an increase in the number of neutrophilic granulocytes but there was also a significant increase in eosinophilic granulocytes. There was also a slight but significant increase in the lymphocyte count in the bronchoalveolar lavage fluid of patients with Wegener's granulomatosis. The number of CD-3-positive lymphocytes was significantly higher than with the controls. No significant differences were noted for the CD-4-positive, CD-8-positive and CD-19-positive lymphocytes. Immunophenotyping of the alveolar macrophages with monoclonal differentiation markers of the Ki-M-Series showed that the alveolar macrophages of patients with Wegener's granulomatosis resembled an immunophenotype that was close to that of monocytes. The number of proliferating macrophages was also higher than with the controls.
对14例经组织学确诊为韦格纳肉芽肿且有肺部受累X线征象的患者进行了支气管肺泡灌洗。将韦格纳肉芽肿患者淋巴细胞和肺泡巨噬细胞的细胞分布及免疫表型特征与10名对照者的检查结果进行比较。肉芽肿患者的支气管肺泡灌洗显示总细胞数显著增加，表明肺部有炎症累及。细胞分类学分析显示，韦格纳肉芽肿患者灌洗液中粒细胞总数显著增加（1例高达40%）。与对照组相比，粒细胞计数中位数增加了五倍。粒细胞计数增加主要是由于嗜中性粒细胞数量增加，但嗜酸性粒细胞数量也显著增加。韦格纳肉芽肿患者支气管肺泡灌洗液中的淋巴细胞计数也有轻微但显著的增加。CD-3阳性淋巴细胞数量显著高于对照组。CD-4阳性、CD-8阳性和CD-19阳性淋巴细胞未见显著差异。用Ki-M系列单克隆分化标志物对肺泡巨噬细胞进行免疫表型分析显示，韦格纳肉芽肿患者的肺泡巨噬细胞类似于接近单核细胞的免疫表型。增殖巨噬细胞的数量也高于对照组。
