[Polymorphism of prolactin in amniotic fluid of women at various times of pregnancy].

The level and molecular forms of prolactin (PRL) in amniotic fluid of pregnant women were investigated at various time of pregnancy with the help of radioimmunoassay, electrophoresis in polyacrylamide gel and immune blotting technique using poly- and monoclonal antibodies to human hypophyseal prolactin (PRL). Changes in the level of immunoreactive PRL in amniotic fluid had the shape of a Gaussian curve with a maximum at 22-29 weeks. Amniotic PRL was characterized by molecular polymorphism. A conclusion has been made that amniotic fluid PRL in pregnancy presents an independent pool of hormones, unrelated to hypophyseal PRL.