人类血清和羊水催乳素的异质性。

Heterogeneity of serum and amniotic fluid prolactin in humans.

作者信息

Fukuoka H, Hamamoto R, Higurashi M

机构信息

Department of Maternal and Child Health, School of Public Health, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Horm Res. 1991;35 Suppl 1:58-63. doi: 10.1159/000181931.

DOI:10.1159/000181931

PMID:1752608

Abstract

The molecular heterogeneity of PRL was studied in serum and amniotic fluid using immunoperoxidase electrophoresis. In the amniotic fluid, larger molecular variants (greater than 117 kD), small PRL (20 and 23 kD) and cleaved PRL were present in the nonreduced condition. Newly, three mercaptoethanol (MCE)-resistant forms (76, 64 and 53 kD), and 25- and 23-kD forms appeared after treatment with MCE. Two variants, 64 and 23 kD, did not bind to concanavalin A (Con A), indicating a simple peptide without sugars. Other glycosylated forms, 76, 53 and 25 kD, bound to Con A. In maternal serum, the same components as those in the amniotic fluid were seen, except for a scanty amount of 23 kD and a newly appearing glycosylated variant of 28 kD in the reduced condition. This 23-kD form also appeared with the administration of TRH in women.

摘要

采用免疫过氧化物酶电泳法研究了血清和羊水中催乳素（PRL）的分子异质性。在羊水中，在非还原条件下存在较大分子变体（大于117 kD）、小分子PRL（20和23 kD）以及裂解的PRL。最近，在用巯基乙醇（MCE）处理后出现了三种抗MCE形式（76、64和53 kD）以及25 kD和23 kD形式。两种变体，64 kD和23 kD，不与伴刀豆球蛋白A（Con A）结合，表明是无糖的简单肽。其他糖基化形式，76 kD、53 kD和25 kD，与Con A结合。在母体血清中，除了还原条件下少量的23 kD和新出现的28 kD糖基化变体之外，可见到与羊水中相同的成分。这种23 kD形式在女性注射促甲状腺激素释放激素（TRH）时也会出现。