应用TaqMan探针实时荧光定量PCR技术对AKAP10基因2073A/G单核苷酸多态性进行基因分型

[Genotyping of AKAP10 gene 2073A/G single nucleotide polymorphism by TaqMan probe real-time PCR].

作者信息

Wang Mo-jin, Zhou Zong-guang, Wang Ling, Li Yuan, Zhang Peng, Zhang Yi, Cui Chang-fu, Zhou Bin

机构信息

Institute of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Mar;40(2):275-8.

PMID:19462906

Abstract

OBJECTIVE

To detect AKAP10 gene 2073A/G single nucleotide polymorphism (SNP) genotyping by TaqMan probe real-time PCR.

METHODS

The genotype of AKAP10 gene 2073A/G was detected by real-time PCR with a pair of new-designed TaqMan probes. The PCR products also were subjected to gene sequence analysis to validate the results of TaqMan probe real-time PCR.

RESULTS

The TaqMan probe real-time PCR method was successfully developed to detect AKAP10 gene 2073A/G SNP. The results were accordant with those achieved by DNA sequencing. The distribution of AKAP10 gene 2073A/G among population had no relationship with gender, age (P > 0.05). Unconditional logistic regression analysis revealed that the variant genotypes (AG + GG) had a 52% increased risk of colorectal cancer, compared with the AA genotype (P = 0.019).

CONCLUSION

A detection platform for SNP genotyping by TaqMan probe was set up successfully. There was a significant association between AKAP10 gene 2073A/G and colorectal cancer.

摘要

目的

采用TaqMan探针实时荧光定量PCR技术检测AKAP10基因2073A/G单核苷酸多态性（SNP）的基因分型。

方法

使用一对新设计的TaqMan探针通过实时荧光定量PCR检测AKAP10基因2073A/G的基因型。PCR产物还进行了基因序列分析以验证TaqMan探针实时荧光定量PCR的结果。

结果

成功建立了用于检测AKAP10基因2073A/G SNP的TaqMan探针实时荧光定量PCR方法。结果与DNA测序结果一致。AKAP10基因2073A/G在人群中的分布与性别、年龄无关（P>0.05）。非条件logistic回归分析显示，与AA基因型相比，变异基因型（AG+GG）患结直肠癌的风险增加52%（P=0.019）。