Wang Li-li, Gao Qing-hong, Zheng Gen-jian, Wang Xiao-yi, Zhou Lan, Wang Chang-mei, Wen Yu-ming
Laboratory of Oral Diseases, Department of Head and Neck Oncology, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Mar;40(2):279-83.
To investigate the embolization effect of Pingyangmycin-albumin microspheres (PYM-AMS) on small arteries and its process of degradation in vivo.
Twenty four Japanese white rabbits were randomly divided into four groups, 6 in each group. PYM hydrochloride + 0.9% NaCl, PYM + soybean oil, and PYM-AMS + soybean oil were injected into the central auricular arteries of the rabbits in the three experimental groups, respectively, for about 30 seconds (0.26 mL/per ear, which contained PYM 5 mg/mL). The vessel samples were taken and examined at 2, 7, 14, and 21 days.
The PYM + 0.9% NaCl group had no significant vessel changes. In the PYM+ soybean oil group, some endothelial cells dropped off at the 7th day after injection. At the 21st day, mild proliferation of endothelial cells and walls of central auricular arteries were observed, especially on the intima. But the lumen was still obvious and the blood flow was not blocked. In the PYM-AMS group, the central auricular arteries were narrowed at the 7th day after injection. At the 21st day, the vessels had sclerostenosis, and the blood flow was blocked. At the 14th day, significant proliferation of endothelial cells and walls of central auricular arteries were observed. The surface of PYM-AMS was absorbed. At the 21st day, the walls of central auricular arteries and some small veins proliferated obviously, and the arteries were sclerostenosed. Many smooth muscle cells, fibroblasts, and myofibroblasts in the original blood vessel lumen appeared. There were thrombi besides the PYM-AMS.
PYM-AMS may become an option for the treatment of large venous or arteriovenous malformations and for the local chemotherapy of malignant tumors.
探讨平阳霉素白蛋白微球（PYM-AMS）对小动脉的栓塞效果及其体内降解过程。
将24只日本大耳白兔随机分为4组，每组6只。分别向3个实验组家兔的耳中央动脉内注入盐酸平阳霉素+0.9%氯化钠、平阳霉素+大豆油、平阳霉素白蛋白微球+大豆油，持续约30秒（0.26 mL/耳，含平阳霉素5 mg/mL）。分别于2、7、14和21天取材并进行血管标本检查。
平阳霉素+0.9%氯化钠组血管无明显变化。平阳霉素+大豆油组，注射后第7天部分内皮细胞脱落。第21天，可见耳中央动脉内皮细胞及血管壁轻度增生，以内膜明显。但管腔仍明显，血流未阻断。平阳霉素白蛋白微球组，注射后第7天耳中央动脉变窄。第21天，血管出现硬化性狭窄，血流阻断。第14天，可见耳中央动脉内皮细胞及血管壁明显增生。平阳霉素白蛋白微球表面被吸收。第21天，耳中央动脉壁及部分小静脉明显增生，动脉出现硬化性狭窄。原血管腔内出现许多平滑肌细胞、成纤维细胞和肌成纤维细胞。除平阳霉素白蛋白微球外还有血栓形成。
平阳霉素白蛋白微球可能成为治疗大静脉或动静脉畸形以及恶性肿瘤局部化疗的一种选择。
