磷脂酰肌醇-3,4,5-三磷酸相互作用组

PI(3,4,5)P3 Interactome.

作者信息

Catimel Bruno, Yin Meng-Xin, Schieber Christine, Condron Melanie, Patsiouras Heather, Catimel Jenny, Robinson Diane E J E, Wong Leon S-M, Nice Edouard C, Holmes Andrew B, Burgess Antony W

机构信息

Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, and School of Chemistry, Bio21 Institute, University of Melbourne, Parkville, Victoria 3010, Australia.

出版信息

J Proteome Res. 2009 Jul;8(7):3712-26. doi: 10.1021/pr900320a.

DOI:10.1021/pr900320a

PMID:19463016

Abstract

Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC-MS/MS experiments.

摘要

将化学合成的PI(3,4,5)P3类似物固定在Affi-10磁珠上，并将其整合到脂质体中，这使得它们能够作为亲和吸附剂，用于利用LIM1215结肠癌细胞系的胞质细胞提取物对磷酸肌醇相互作用组进行全面分析。这导致鉴定出282种蛋白质，这些蛋白质要么与PI(3,4,5)P3相互作用，要么作为包含PI(3,4,5)P3结合伴侣的复合物的一部分被间接捕获。这些蛋白质是通过亲和/液相色谱-串联质谱实验鉴定出来的。