磷脂酰肌醇-3,5-二磷酸（PI(3,5)P2）和磷脂酰肌醇-4,5-二磷酸（PI(4,5)P2）相互作用组
The PI(3,5)P2 and PI(4,5)P2 interactomes.
作者信息
Catimel Bruno, Schieber Christine, Condron Melanie, Patsiouras Heather, Connolly Lisa, Catimel Jenny, Nice Edouard C, Burgess Antony W, Holmes Andrew B
机构信息
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Parkville Victoria 3052, Australia.
出版信息
J Proteome Res. 2008 Dec;7(12):5295-313. doi: 10.1021/pr800540h.
A comprehensive analysis of the phosphoinositide interactome has been performed using analogues of PI(3,5)P2 and PI(4,5)P2 phosphatidyl phospholipids which were immobilized onto Affi-10 beads or incorporated into liposomes for use as affinity absorbents with cytosolic extracts from colonic carcinoma cell lines. Affinity/LC/MS/MS experiments allowed identification of 388 proteins/protein complexes that appeared to interact specifically with the phosphoinositide targets: a number of novel potential phosphoinositide interacting proteins have been identified.
利用固定在Affi - 10磁珠上或掺入脂质体中的PI(3,5)P2和PI(4,5)P2磷脂类似物作为亲和吸附剂，与结肠癌细胞系的胞质提取物一起，对磷酸肌醇相互作用组进行了全面分析。亲和/液相色谱/串联质谱实验鉴定出388种似乎与磷酸肌醇靶点特异性相互作用的蛋白质/蛋白质复合物：已鉴定出一些新的潜在磷酸肌醇相互作用蛋白。
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