Toh H
Protein Engineering Research Institute, Osaka, Japan.
Protein Seq Data Anal. 1991 Aug;4(2):111-7.
A conservative sequence motif was extracted from an alignment of the firefly luciferase family. AngR derived from a pathogenic bacterium and acetyl-CoA synthetases derived from two ascomycete fungi were identified as members of the firefly luciferase family by a homology search with the motif and other sequence comparison analyses. The motif sequence shares several characteristics with the phosphate-binding sites of phosphoproteins and nucleotide-binding proteins. A multiple alignment and an unrooted phylogenetic tree were constructed for the investigation of evolutionary relationships within the firefly luciferase family.
从萤火虫荧光素酶家族的比对中提取了一个保守序列基序。通过与该基序的同源性搜索和其他序列比较分析，鉴定出源自致病细菌的AngR和源自两种子囊菌的乙酰辅酶A合成酶为萤火虫荧光素酶家族的成员。该基序序列与磷蛋白和核苷酸结合蛋白的磷酸结合位点具有若干共同特征。构建了一个多序列比对和一个无根系统发育树，以研究萤火虫荧光素酶家族内部的进化关系。
