Toh H
Protein Engineering Research Institute, Osaka, Japan.
Protein Seq Data Anal. 1990 Dec;3(6):517-21.
It was found, by computer-assisted homology search, that the N-terminal halves of gramicidin S synthetase 1 and tyrocidine synthetase 1 are homologous with beetle luciferases and plant 4-coumarate:CoA ligases. The comparison of the reactions catalyzed by these enzymes showed that they are involved in similar reactions; the adenylation of their substrates and the formation of thiolester. Structural and functional implication of the sequence homology and molecular evolution of these proteins are discussed.
通过计算机辅助同源性搜索发现，短杆菌肽S合成酶1和短杆菌酪肽合成酶1的N端半段与甲虫荧光素酶和植物4-香豆酸：辅酶A连接酶同源。对这些酶催化的反应进行比较表明，它们参与相似的反应；使底物腺苷化并形成硫酯。讨论了这些蛋白质序列同源性和分子进化的结构与功能意义。
