Rober M, Walter J, Vlakh E, Stahl F, Kasper C, Tennikova T
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia.
Anal Chim Acta. 2009 Jun 30;644(1-2):95-103. doi: 10.1016/j.aca.2009.04.015. Epub 2009 Apr 17.
Polymer macroporous monoliths are widely used as efficient sorbents in different, mostly dynamic, interphase processes. In this paper, monolithic materials strongly bound to the inert glass surface are suggested as operative matrices at the development of three-dimensional (3-D) microarrays. For this purpose, several rigid macroporous copolymers differed by reactivity and hydrophobic-hydrophilic properties were synthesized and tested: (1) glycidyl methacrylate-co-ethylene dimethacrylate (poly(GMA-co-EDMA)), (2) glycidyl methacrylate-co-glycerol dimethacrylate (poly(GMA-co-GDMA)), (3) N-hydroxyphthalimide ester of acrylic acid-co-glycidyl methacrylate-co-ethylene dimethacrylate (poly(HPIEAA-co-GMA-co-EDMA)), (4) 2-cyanoethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-EDMA)), and (5) 2-cyanoethyl methacrylate-co-2-hydroxyethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-HEMA-co-EDMA)). The constructed devices were used as platforms for protein microarrays construction and model mouse IgG-goat anti-mouse IgG affinity pair was used to demonstrate the potential of developed test-systems, as well as to optimize microanalytical conditions. The offered microarray platforms were applied to detect the bone tissue marker osteopontin directly in cell culture medium.
聚合物大孔整体柱作为高效吸附剂广泛应用于不同的、主要是动态的相间过程中。本文提出将与惰性玻璃表面紧密结合的整体材料作为三维（3-D）微阵列开发中的操作基质。为此，合成并测试了几种在反应性和疏水-亲水性方面存在差异的刚性大孔共聚物：（1）甲基丙烯酸缩水甘油酯-二甲基丙烯酸乙二酯共聚物（聚（GMA-co-EDMA）），（2）甲基丙烯酸缩水甘油酯-二甲基丙烯酸甘油酯共聚物（聚（GMA-co-GDMA）），（3）丙烯酸N-羟基邻苯二甲酰亚胺酯-甲基丙烯酸缩水甘油酯-二甲基丙烯酸乙二酯共聚物（聚（HPIEAA-co-GMA-co-EDMA）），（4）甲基丙烯酸2-氰基乙酯-二甲基丙烯酸乙二酯共聚物（聚（CEMA-co-EDMA）），以及（5）甲基丙烯酸2-氰基乙酯-甲基丙烯酸2-羟乙酯-二甲基丙烯酸乙二酯共聚物（聚（CEMA-co-HEMA-co-EDMA））。构建的装置用作蛋白质微阵列构建的平台，并使用模型小鼠IgG-山羊抗小鼠IgG亲和对来证明所开发测试系统的潜力，以及优化微分析条件。所提供的微阵列平台用于直接在细胞培养基中检测骨组织标志物骨桥蛋白。
