Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia.
Talanta. 2012 May 15;93:139-46. doi: 10.1016/j.talanta.2012.01.064. Epub 2012 Feb 7.
Macroporous monoliths with different surface functionalization (reactive groups) were utilized as platforms for DNA analysis in microarray format. The slides based on a copolymer glycidyl methacrylate-co-ethylene dimethacrylate (GMA-EDMA) have been chosen as well known and thoroughly studied standard. In particular, this material has been used at optimization of DNA microanalytical procedure. The concentration and pH of spotting solution, immobilization temperature and time, blocking agent and coupling reaction duration were selected as varied parameters. The efficiency of analysis performed on 3-D monolithic platforms was compared to that established for commercially available glass slides. As a practical example, a diagnostic test for detection of CFTR gene mutation was carried out. Additionally, the part of presented work was devoted to preparation of aptamer-based test-system that allowed successful and highly sensitive detection both of DNA and protein.
采用具有不同表面功能化（反应基团）的大孔整体材料作为微阵列格式的 DNA 分析平台。选择基于甲基丙烯酸缩水甘油酯-共-乙二醇二甲基丙烯酸酯（GMA-EDMA）共聚物的载玻片作为众所周知且经过充分研究的标准。特别是，该材料已用于 DNA 微分析程序的优化。点样溶液的浓度和 pH 值、固定化温度和时间、封闭剂和偶联反应持续时间被选为变化参数。在 3-D 整体平台上进行的分析效率与商业上可用的玻璃载玻片上建立的效率进行了比较。作为一个实际的例子，进行了用于检测 CFTR 基因突变的诊断测试。此外，本工作的一部分致力于制备基于适体的测试系统，该系统允许成功且高度敏感地检测 DNA 和蛋白质。
