Kim R S, Zaborniak C L
Department of Pharmacology and Therapeutics, University of Manitoba, Faculty of Medicine, Winnipeg, Canada.
Prostaglandins Leukot Essent Fatty Acids. 1991 Aug;43(4):223-7. doi: 10.1016/0952-3278(91)90034-3.
The cytotoxic effect of aldehydic metabolites of linoleic acid, 13-oxo-tridecadienoic acids, on MCF-7 human breast cancer cells was investigated. The metabolites inhibited the growth of the cancer cells and the effect was dependent on both time of exposure and concentration of the metabolites; 50% growth inhibition occurred at approximately 55 and 33 microM, after 3- and 5-day incubations, respectively. The metabolites had greater cytotoxicity than parent linoleic acid or other polyunsaturated fatty acids tested. The antiproliferative effect was partially reversed by 10 microM of dithiothreitol suggesting that attack on thiol groups in cancer cells by highly reactive alpha, beta-unsaturated carbonyl moiety in the metabolites was responsible for the cytotoxic actions.
研究了亚油酸的醛代谢产物13-氧代-十三碳二烯酸对MCF-7人乳腺癌细胞的细胞毒性作用。这些代谢产物抑制癌细胞的生长，且该作用取决于暴露时间和代谢产物的浓度；在分别培养3天和5天后，浓度约为55 microM和33 microM时出现50%的生长抑制。这些代谢产物比母体亚油酸或所测试的其他多不饱和脂肪酸具有更强的细胞毒性。10 microM的二硫苏糖醇可部分逆转其抗增殖作用，这表明代谢产物中高反应性的α,β-不饱和羰基部分对癌细胞中硫醇基团的攻击是细胞毒性作用的原因。
