发育生物学中的选择性平面照明显微技术。
Selective plane illumination microscopy techniques in developmental biology.
作者信息
Huisken Jan, Stainier Didier Y R
机构信息
Department of Biochemistry and Biophysics, and Cardiovascular Research Institute, University of California, San Francisco, CA 94158, USA.
出版信息
Development. 2009 Jun;136(12):1963-75. doi: 10.1242/dev.022426.
Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.
选择性平面照明显微镜（SPIM）以及其他使用聚焦光片照射样本的荧光显微镜技术在发育研究中越来越受欢迎。荧光光片显微镜弥补了荧光立体显微镜与固定组织切片高分辨率成像之间图像质量的差距。此外，高深度穿透、低漂白和高采集速度使光片显微镜非常适合在活胚胎中进行长时间延时实验。本文综述将光片显微镜与共聚焦显微镜等成熟的荧光显微镜技术的优缺点进行了比较，并讨论了这种易于应用的技术的不同实现方式和应用。
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