Kramer Stephanie N, Antarasen Jeanpun, Reinholt Cole R, Kisley Lydia
Department of Physics, Case Western Reserve University, Rockefeller Building, 2076 Adelbert Road, Cleveland, Ohio 44106, USA.
J Appl Phys. 2024 Sep 7;136(9):091101. doi: 10.1063/5.0218262. Epub 2024 Sep 4.
We present a comprehensive guide to light-sheet microscopy (LSM) to assist scientists in navigating the practical implementation of this microscopy technique. Emphasizing the applicability of LSM to image both static microscale and nanoscale features, as well as diffusion dynamics, we present the fundamental concepts of microscopy, progressing through beam profile considerations, to image reconstruction. We outline key practical decisions in constructing a home-built system and provide insight into the alignment and calibration processes. We briefly discuss the conditions necessary for constructing a continuous 3D image and introduce our home-built code for data analysis. By providing this guide, we aim to alleviate the challenges associated with designing and constructing LSM systems and offer scientists new to LSM a valuable resource in navigating this complex field.
我们提供了一份光片显微镜（LSM）的综合指南，以帮助科学家们实际应用这种显微镜技术。我们强调了LSM在成像静态微观和纳米尺度特征以及扩散动力学方面的适用性，介绍了显微镜的基本概念，从光束轮廓的考虑到图像重建。我们概述了构建自制系统时的关键实际决策，并深入探讨了对准和校准过程。我们简要讨论了构建连续3D图像所需的条件，并介绍了我们用于数据分析的自制代码。通过提供本指南，我们旨在减轻与设计和构建LSM系统相关的挑战，并为刚接触LSM的科学家们提供在这个复杂领域中导航的宝贵资源。
