Becker K, Jährling N, Kramer E R, Schnorrer F, Dodt H-U
Vienna University of Technology, Institute of Solid State Electronics, Dept. of Bioelectronics, Floragasse 7, 1040 Vienna, Austria.
J Biophotonics. 2008 Mar;1(1):36-42. doi: 10.1002/jbio.200710011.
Ultramicroscopy is a microscopical technique that allows optical sectioning and 3D reconstruction of biological and medical specimens. While in confocal microscopy specimen size is limited to several hundred micrometers at best, using ultramicroscopy even centimeter sized objects like whole mouse embryos can be reconstructed with micrometer resolution. This is possible by using a combination of a clearing procedure and the principle of lightsheet illumination. We present ultramicroscopic 3D reconstructions of whole immunohistochemically labelled mouse embryos and adult Drosophila, giving detailed insight into their anatomy. Its speed and simplicity makes ultramicroscopy ideally suited for high-throughput phenotype screening of transgenic mice and thus will benefit the investigation of disease models.
超显微镜是一种显微技术，可对生物和医学标本进行光学切片和三维重建。在共聚焦显微镜中，标本尺寸最大只能限制在几百微米，而使用超显微镜，即使是厘米大小的物体，如完整的小鼠胚胎，也能以微米分辨率进行重建。这可以通过结合一种透明处理程序和光片照明显微技术来实现。我们展示了完整的免疫组化标记小鼠胚胎和成年果蝇的超显微三维重建，深入了解了它们的解剖结构。超显微镜的速度和简便性使其非常适合对转基因小鼠进行高通量表型筛选，从而将有助于疾病模型的研究。
