Kammerman S, Demopoulos R I, Ross J
Cancer Res. 1977 Aug;37(8 Pt 1):2578-82.
Gonadotropic hormones are required for the induction and maintenance of tumors arising in ovaries that have been transplanted to the spleens of gonadectomized mice. The characteristics of gonadotropin receptors for human chorionic gonadotropin (HCG)-luteinizing hormone on cells from these tumors of varying size, age, and morphology have been determined. The specific binding of 125I-labeled HCG to cells obtained by collagenase digestion, 15 to 65 weeks postimplantation from granulosa cell or luteinized cell, or mixed granulosa-luteal tumors was analyzed by Scatchard plot. Neither the size, weight, duration of implantation, nor histological morphology affected the receptor-binding affinity [equilibrium dissociation constant (Kd), 6 X 10(-10) M], and, presumably, the receptor is qualitatively similar. In contrast, the number of HCG receptors per cell increased 17-fold and was related to the degree of morphological luteinization of the tumor. HCG-sensitive adenyl cyclase was also demonstrated and compared to HCG binding in a highly luteinized tumor.
促性腺激素对于诱导和维持移植到性腺切除小鼠脾脏中的卵巢所产生的肿瘤是必需的。已经确定了来自这些大小、年龄和形态各异的肿瘤细胞上的人绒毛膜促性腺激素（HCG）-促黄体生成素促性腺激素受体的特征。通过Scatchard作图分析了在植入后15至65周从颗粒细胞、黄体化细胞或颗粒-黄体混合瘤中通过胶原酶消化获得的细胞对125I标记的HCG的特异性结合。肿瘤的大小、重量、植入持续时间或组织学形态均不影响受体结合亲和力[平衡解离常数（Kd），6×10（-10）M]，并且推测该受体在质量上是相似的。相比之下，每个细胞的HCG受体数量增加了17倍，并且与肿瘤的形态黄体化程度相关。还在一个高度黄体化的肿瘤中证明了HCG敏感的腺苷酸环化酶并将其与HCG结合进行了比较。
