Evans M J, Kovacs C J
Cell Tissue Kinet. 1977 May;10(3):233-43.
Growth and cell proliferation kinetics of hepatoma H-4-II-E and its tissue culture derivative have been studied to establish the characteristics of an in vivo--in vitro solid tumor model. The H-4-II-E line, originating from the Reuber H-35 hepatoma, can be maintained and studied either in cell culture or as a transplantable solid tumor in ACI male rats. In addition it allows for the in vitro assay of cell survival following treatment of animal tumors in situ. In vivo, hepatoma H-4-II-E is rapidly growing tumor with a mean doubling time of 49-2 hr. The cell cyle time is 39-1 hr with a cell loss factor of 0-32. Retrospective examination of tumor specimens obtained during the establishment of the H-4-II-E tumor system demonstrates that both structural as well as cell population changes have occurred. The biological characteristics of the primary tumor (H-35) and an early intermediate stage (H-35tc2) are compared with H-4-II-E and the histopathological, growth and cell kinetic changes are discussed.
对肝癌H-4-II-E及其组织培养衍生物的生长和细胞增殖动力学进行了研究，以确定一种体内-体外实体瘤模型的特征。源自鲁伯H-35肝癌的H-4-II-E细胞系，可以在细胞培养中维持和研究，也可以作为可移植实体瘤在ACI雄性大鼠体内进行研究。此外，它还允许对原位治疗动物肿瘤后的细胞存活进行体外测定。在体内，肝癌H-4-II-E是一种快速生长的肿瘤，平均倍增时间为49-2小时。细胞周期时间为39-1小时，细胞丢失率为0-32。对在H-4-II-E肿瘤系统建立过程中获得的肿瘤标本进行回顾性检查表明，结构和细胞群体都发生了变化。将原发性肿瘤（H-35）和早期中间阶段（H-35tc2）的生物学特性与H-4-II-E进行比较，并讨论了组织病理学、生长和细胞动力学变化。
