Deutman Alexander B C, Monnereau Cyrille, Moalin Mohamed, Coumans Ruud G E, Veling Nico, Coenen Michiel, Smits Jan M M, de Gelder René, Elemans Johannes A A W, Ercolani Gianfranco, Nolte Roeland J M, Rowan Alan E
Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands.
Proc Natl Acad Sci U S A. 2009 Jun 30;106(26):10471-6. doi: 10.1073/pnas.0810145106. Epub 2009 May 21.
The cooperative binding effects of viologens and pyridines to a synthetic bivalent porphyrin receptor are used as a model system to study how the magnitudes of these effects relate to the experimentally obtained values. The full thermodynamic and kinetic circles concerning both activation and inhibition of the cage of the receptor for the binding of viologens were measured and evaluated. The results strongly emphasize the apparent character of measured binding and rate constants, in which the fractional saturation of receptors with other guests is linearly expressed in these constants. The presented method can be used as a simple tool to better analyze and comprehend the experimentally observed kinetics and thermodynamics of natural and artificial cooperative systems.
将紫精和吡啶与合成二价卟啉受体的协同结合效应用作模型系统，以研究这些效应的大小如何与实验获得的值相关。测量并评估了与紫精结合相关的受体笼激活和抑制的完整热力学和动力学循环。结果强烈强调了所测结合常数和速率常数的表观特征，其中受体与其他客体的分数饱和度在这些常数中呈线性表达。所提出的方法可作为一种简单工具，用于更好地分析和理解天然和人工协同系统中实验观察到的动力学和热力学。
