粗茎鳞毛蕨中两种间苯三酚类化合物——黄绵马酸AB和PB的抗菌活性
Antibacterial activity of two phloroglucinols, flavaspidic acids AB and PB, from Dryopteris crassirhizoma.
作者信息
Lee Hyang Burm, Kim Jin Cheol, Lee Sang Myung
机构信息
College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, Korea.
出版信息
Arch Pharm Res. 2009 May;32(5):655-9. doi: 10.1007/s12272-009-1502-9. Epub 2009 May 27.
The antimicrobial effect of solvent extracts from the rhizome of a thick-stemmed wood fern (Dryopteris crassirhizoma) was evaluated and its phloroglucinol components, flavaspidic acids PB and AB. Flavaspidic acids PB and AB were isolated from the D. crassirhizoma rhizomes by methanol extraction, followed by silica gel and Sephadex LH-20 column chromatography. The chemical structures were characterized by spectral techniques, including ESI-MS, UV, (1)H- and (13)C-NMR spectrum analysis. When the antimicrobial activity of the extracts and compounds was tested by the paper disc method, the extracts and compounds were highly active against Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus KCTC 1928 (a MRSA bacterium), Streptococcus mutans and Bacillus subtilis. The extracts and compounds were not active against fungi and chlorella. Our study revealed that the antibacterial activity of samples from D. crassirhizoma was mainly related to the flavaspidic acids.
对粗茎鳞毛蕨（Dryopteris crassirhizoma）根茎的溶剂提取物的抗菌效果进行了评估，及其间苯三酚成分、黄绵马酸PB和AB。通过甲醇提取，然后进行硅胶和葡聚糖凝胶LH - 20柱色谱法，从粗茎鳞毛蕨根茎中分离出黄绵马酸PB和AB。通过光谱技术对化学结构进行了表征，包括电喷雾电离质谱（ESI - MS）、紫外光谱（UV）、氢核磁共振（¹H - NMR）和碳核磁共振（¹³C - NMR）光谱分析。当通过纸片法测试提取物和化合物的抗菌活性时，提取物和化合物对革兰氏阳性菌具有高度活性，如耐甲氧西林金黄色葡萄球菌KCTC 1928（一种耐甲氧西林金黄色葡萄球菌）、变形链球菌和枯草芽孢杆菌。提取物和化合物对真菌和小球藻没有活性。我们的研究表明，粗茎鳞毛蕨样品的抗菌活性主要与黄绵马酸有关。
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