Jiang Yong-Guang, Zhao Jia-Hui, Luo Yong, He Da-Lin, Wang Xin-Yang, Cui Xin-Hao
Department of Urology, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing 100029, China.
Zhonghua Nan Ke Xue. 2009 Apr;15(4):310-3.
To determine the Wnt/beta-catenin signal pathway in different human prostate cancer cell lines and explore its role in epithelial-mesenchymal transition (EMT) in human prostate cancer.
We detected the expressions of beta-catenin, t-GSK3beta and p-GSK3beta in several prostate cancer cell lines (LNCaP, C4, C4-2, C4-2B, IF11, IA8, PC-3 and DU145) with different characteristics of epithelial-mesenchymal transition (EMT) by Western blotting.
There were remarkable differences in the expressions of beta-catenin and p-GSK3beta among the cell lines, with beta-catenin and p-GSK3beta highly expressed in LNCaP, C4, C4-2, C4-2B, IF11 and IA8, lowly expressed in PC-3 and DU145, but no difference was observed in the expressions of t-GSK3beta in all the cell lines.
There are differences in the state of the Wnt/beta-catenin signal pathway among the cell lines with different characters of EMT.
确定不同人前列腺癌细胞系中的Wnt/β-连环蛋白信号通路，并探讨其在人前列腺癌上皮-间质转化（EMT）中的作用。
我们通过蛋白质印迹法检测了几种具有不同上皮-间质转化（EMT）特征的前列腺癌细胞系（LNCaP、C4、C4-2、C4-2B、IF11、IA8、PC-3和DU145）中β-连环蛋白、t-GSK3β和p-GSK3β 的表达。
各细胞系中β-连环蛋白和p-GSK3β 的表达存在显著差异，β-连环蛋白和p-GSK3β 在LNCaP、C4、C4-2、C4-2B、IF11和IA8中高表达，在PC-3和DU145中低表达，但所有细胞系中t-GSK3β 的表达无差异。
具有不同EMT特征的细胞系中Wnt/β-连环蛋白信号通路的状态存在差异。
