Wu Kai-Jie, Zhang Dong, Zhu Guang-Feng, Cheng He-Peng, Zeng Jin, Zhang Lin-Lin, Wang Xin-Yang, He Da-Lin
Department of Urology, The First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China.
Zhonghua Nan Ke Xue. 2009 Jan;15(1):41-4.
To observe the expression of Smad4, the core of TGF-beta/Smads signal transduction pathway in different prostate cancer cell lines, and explore their molecular mechanism of bone metastatic potential.
The Millicell polycarbonate filter coated with matrigel was used to confirm the invasive potency of LNCaP and ARCaP cell lines (IF11 and IA8). The expressions of the Smad4 protein and mRNA in these prostate cancer cells with different metastatic potentials were detected by Western blotting and RT-PCR, respectively.
ARCaP cell lines (IF11 and IA8) exhibited a stronger potency of invasion than LNCaP (P < 0.01). The Smad4 protein and mRNA highly expressed in the LNCaP cell line that was well-known with a low metastatic potential, but not in the ARCaP (IF11 or IA8) cells with high metastatic potentials (P < 0.01).
Smad4 expresses differently in LNCaP and ARCaP cell lines with different metastatic potentials and, as a tumor suppressive gene, its deficient expression may play an important role in the invasion and metastasis of advanced prostate cancer.
观察TGF-β/Smads信号转导通路核心分子Smad4在不同前列腺癌细胞系中的表达情况，探讨其骨转移潜能的分子机制。
采用铺有基质胶的密理博聚碳酸酯滤膜检测LNCaP和ARCaP细胞系（IF11和IA8）的侵袭能力。分别通过蛋白质免疫印迹法和逆转录聚合酶链反应检测不同转移潜能的前列腺癌细胞中Smad4蛋白和mRNA的表达。
ARCaP细胞系（IF11和IA8）的侵袭能力强于LNCaP（P<0.01）。Smad4蛋白和mRNA在转移潜能低的LNCaP细胞系中高表达，而在转移潜能高的ARCaP（IF11或IA8）细胞中不表达（P<0.01）。
Smad4在具有不同转移潜能的LNCaP和ARCaP细胞系中表达不同，作为一种肿瘤抑制基因，其表达缺失可能在晚期前列腺癌的侵袭和转移中起重要作用。
