Pauliukaite Rasa, Ghica Mariana Emilia, Fatibello-Filho Orlando, Brett Christopher M A
Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, 3004-535 Coimbra, Portugal.
Anal Chem. 2009 Jul 1;81(13):5364-72. doi: 10.1021/ac900464z.
The effectiveness of immobilization of functionalized carbon nanotubes into chitosan using different cross-linking agents has been evaluated. The cross-linkers used were glyoxal (GO), glutaraldehyde (GA), epichlorohydrin (ECH), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide together with N-hydroxysuccinimide (EDC-NHS), and the nanotubes were retained on graphite epoxy resin composite electrodes. The nanotube modified electrodes have been characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Using CV and EIS in the presence of potassium hexacyanoferrate(III), the electroactive area of all types of electrodes was determined and the redox process analyzed, leading to the conclusion that ECH and EDC-NHS are better for immobilization of functionalized carbon nanotubes inside the chitosan matrix. The modified electrodes were successfully applied to the determination of hydrogen peroxide by fixed potential amperometry at -0.1 V vs SCE, the highest response being exhibited when using ECH.
评估了使用不同交联剂将功能化碳纳米管固定在壳聚糖中的有效性。所使用的交联剂有乙二醛（GO）、戊二醛（GA）、环氧氯丙烷（ECH）以及1-乙基-3-（3-二甲基氨基丙基）碳二亚胺与N-羟基琥珀酰亚胺（EDC-NHS），并且纳米管被保留在石墨环氧树脂复合电极上。纳米管修饰电极已通过循环伏安法（CV）和电化学阻抗谱（EIS）进行了表征。在铁氰化钾（III）存在的情况下使用CV和EIS，测定了所有类型电极的电活性面积并分析了氧化还原过程，得出结论：ECH和EDC-NHS更有利于将功能化碳纳米管固定在壳聚糖基质内部。修饰电极通过固定电位安培法在相对于饱和甘汞电极（SCE）为-0.1 V的电位下成功应用于过氧化氢的测定，使用ECH时表现出最高响应。
