Faculty of Chemistry, Jagiellonian University, Kraków, Poland.
J Mater Sci Mater Med. 2012 Aug;23(8):1991-2000. doi: 10.1007/s10856-012-4666-7. Epub 2012 May 9.
Novel polymeric hydrogel scaffolds for corneal epithelium cell culturing based on blends of chitosan with some other biopolymers such as hydroxypropylcellulose, collagen and elastin crosslinked with genipin, a natural substance, were prepared. Physicochemical and biomechanical properties of these materials were determined. The in vitro cell culture experiments with corneal epithelium cells have indicated that a membrane prepared from chitosan-collagen blend (Ch-Col) provided the regular stratified growth of the epithelium cells, good surface covering and increased number of the cell layers. Ch-Col membranes are therefore the most promising material among those studied. The performance of Ch-Col membranes is comparable with that of the amniotic membrane which is currently recommended for clinical applications.
基于壳聚糖与羟丙纤维素、胶原和弹性蛋白等其他生物聚合物的混合物，制备了用于角膜上皮细胞培养的新型聚合水凝胶支架，这些聚合物通过天然物质京尼平交联。测定了这些材料的物理化学和生物力学性能。体外角膜上皮细胞培养实验表明，由壳聚糖-胶原混合物（Ch-Col）制备的膜可促进上皮细胞的规则分层生长、良好的表面覆盖和增加细胞层数。因此，Ch-Col 膜是研究中最有前途的材料之一。Ch-Col 膜的性能可与目前推荐用于临床应用的羊膜相媲美。
