Maspin缺失是口腔鳞状细胞癌侵袭和转移的负性预后因素。
Loss of maspin is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma.
作者信息
Yoshizawa Kunio, Nozaki Shinichi, Okamune Ayako, Kitahara Hiroko, Ohara Teruhisa, Kato Koroku, Kawashiri Shuichi, Yamamoto Etsuhide
机构信息
Department of Oral & Maxillofacial Surgery, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan.
出版信息
J Oral Pathol Med. 2009 Jul;38(6):535-9. doi: 10.1111/j.1600-0714.2009.00762.x. Epub 2009 Mar 13.
OBJECTIVE
Maspin, a 42-kDa protein, belongs to the serpin family of protease inhibitors and is known to have tumor-suppressor function. In this study, we investigated the interrelationship between clinicopathologic findings and maspin expression in oral squamous cell carcinoma (OSCC).
METHODS
Using immunohistochemical techniques to examine the expression levels of maspin in OSCC, maspin expression in OSCC was detected in 46 (64.8%) of 71 cases. We also compared the clonicopathologic features of OSCC cases with maspin expression levels. Moreover, we examined expression of maspin in eight cell lines derived from OSCC using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting.
RESULTS
There was a significant correlation between decreased maspin expression and T-category (P < 0.01), lymph metastasis (P < 0.0001), and mode of invasion (P < 0.0001). Patients with positive maspin expression had a significantly better prognosis (P < 0.001). Lower expression of maspin was also seen in cell lines derived from grade 4D, which shows stronger invasive potential than other grades of OSCC.
CONCLUSION
Maspin may be a useful marker to identify the potential for progression in OSCC.
目的
Maspin是一种42 kDa的蛋白质，属于丝氨酸蛋白酶抑制剂的丝氨酸蛋白酶抑制剂家族，已知具有肿瘤抑制功能。在本研究中，我们调查了口腔鳞状细胞癌（OSCC）的临床病理特征与maspin表达之间的相互关系。
方法
采用免疫组织化学技术检测OSCC中maspin的表达水平，在71例病例中的46例（64.8%）检测到OSCC中maspin的表达。我们还比较了具有maspin表达水平的OSCC病例的克隆病理特征。此外，我们使用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法检测了源自OSCC的8种细胞系中maspin的表达。
结果
maspin表达降低与T分期（P < 0.01）、淋巴结转移（P < 0.0001）和侵袭方式（P < 0.0001）之间存在显著相关性。maspin表达阳性的患者预后明显更好（P < 0.001）。在源自4D级的细胞系中也观察到maspin表达较低，4D级显示出比其他等级的OSCC更强的侵袭潜力。
结论
Maspin可能是一种有用的标志物，用于识别OSCC进展的可能性。
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