Tsai Hsiu-Ting, Hsieh Ming-Ju, Lin Chiao-Wen, Su Shih-Chi, Miao Nae-Fang, Yang Shun-Fa, Huang Hui-Chuan, Lai Fu-Chih, Liu Yu-Fan
Accelerated Bachelor of Science in Nursing, College of Nursing, Taipei Medical University, Taipei, Taiwan.
School of Nursing, College of Nursing, Taipei Medical University, Taipei, Taiwan.
PLoS One. 2017 Mar 24;12(3):e0163369. doi: 10.1371/journal.pone.0163369. eCollection 2017.
We identified rs17071138 T/C, rs3744941 C/T, and rs8089104 T/C gene polymorphisms of SERPINB5 (mammary serine protease inhibitor) that are specific to patients with oral cancer susceptibility and their clinicopathological status.
METHODOLOGY/PRINCIPAL FINDINGS: In total, 1342 participants, including 601 healthy controls and 741 patients with oral cancer, were recruited for this study. Allelic discrimination of rs17071138 T/C, rs3744941 C/T, and rs8089104 T/C of the SERPINB5 gene was assessed by a real-time PCR with a TaqMan assay. We found that individuals carrying the polymorphic rs17071138 and rs8089104 are more susceptible to oral cancer (OR, 1.57; 95% CI, 1.072.31 and OR, 1.58; 95% CI, 1.042.39, respectively). Among oral cancer-related risk factor exposures, the individuals carrying the polymorphic rs17071138 had 4.26- (95% CI: 1.6511.01; p = 0.002), 2.34- (95% CI: 1.194.61; p = 0.01), and 2.34-fold (95% CI: 1.38~3.96; p = 0.001) higher risks of developing oral cancer.
Heterozygous TC of the SERPINB5 rs17071138 polymorphism may be a factor that increases susceptibility to oral cancer. Interactions of gene-to-gene and gene-to-oral cancer-related environmental risk factors have a synergetic effect that can further enhance oral cancer development.
我们鉴定出了丝氨酸蛋白酶抑制剂B5（乳腺丝氨酸蛋白酶抑制剂）基因的rs17071138 T/C、rs3744941 C/T和rs8089104 T/C基因多态性，这些多态性与口腔癌易感性患者及其临床病理状态相关。
方法/主要发现：本研究共招募了1342名参与者，包括601名健康对照者和741名口腔癌患者。通过TaqMan实时荧光定量PCR法对丝氨酸蛋白酶抑制剂B5基因的rs17071138 T/C、rs3744941 C/T和rs8089104 T/C进行等位基因鉴别。我们发现，携带多态性rs17071138和rs8089104的个体患口腔癌的易感性更高（OR分别为1.57；95%CI为1.072.31和OR为1.58；95%CI为1.042.39）。在与口腔癌相关的危险因素暴露中，携带多态性rs17071138的个体患口腔癌的风险分别高出4.26倍（95%CI：1.6511.01；p = 0.002）、2.34倍（95%CI：1.194.61；p = 0.01）和2.34倍（95%CI：1.38~3.96；p = 0.001）。
丝氨酸蛋白酶抑制剂B5基因rs17071138多态性的杂合子TC可能是增加口腔癌易感性的一个因素。基因与基因之间以及基因与口腔癌相关环境危险因素之间的相互作用具有协同效应，可进一步促进口腔癌的发生发展。
