Markovic N, Markovic O, Young D S
Clin Chem. 1977 Aug;23(8):1472-5.
We describe a technique whereby it is possible to measure enzyme activity in a singel cell. The model chosen involved the measurement of myeloperoxidase activity in a polymorphonuclear neutrophil leukocyte. Details of the apparatus are described. The experiments necessary to optimize the reaction conditions are summarized. The nature of the phases of the reaction are described. The technique appears to have further application in measurement of the activities of other enzymes in leukocytes or other cells.
我们描述了一种能够测量单个细胞中酶活性的技术。所选用的模型涉及对多形核中性粒细胞中髓过氧化物酶活性的测量。文中描述了该仪器的细节。总结了优化反应条件所需的实验。阐述了反应各阶段的性质。这项技术似乎在测量白细胞或其他细胞中其他酶的活性方面有进一步的应用。
