突尼斯反应性关节炎和未分化关节炎患者的MHC I类和II类基因

MHC class I and class II genes in Tunisian patients with reactive and undifferentiated arthritis.

作者信息

Siala M, Mahfoudh N, Fourati H, Gdoura R, Younes M, Kammoun A, Chour I, Meddeb N, Gaddour L, Hakim F, Baklouti S, Bargaoui N, Sellami S, Hammami A, Makni H

机构信息

Laboratoire de recherche "Micro-organismes et Pathologie Humaine", EPS Habib Bourguiba de Sfax, Tunisia.

出版信息

Clin Exp Rheumatol. 2009 Mar-Apr;27(2):208-13.

PMID:19473559

Abstract

OBJECTIVE

To study HLA class I and class II association in Tunisian patients with reactive (ReA) and undifferentiated arthritis (UA).

METHODS

The study included 17 patients with ReA defined according to the European Spondylarthropathy Study Group criteria for spondylarthropathy (SpA), 11 patients classified as having undifferentiated arthritis and 100 unrelated healthy controls. HLA class I antigens were typed serologically and HLA class II alleles were genotyped molecularly by the polymerase chain reaction with sequence-specific primers technique.

RESULTS

There was a major difference between HLA alleles in ReA and UA patients when compared separately with controls. Increased frequencies of HLA-B27 (p=7.76 10-12, OR=59.30), HLA-B51 (p=0.015, OR=4.91) and HLA-DRB104 (p=0.033, OR=2.90) alleles were found in patients with ReA but not in patients with UA. HLA-B27 was not expressed totally in our cohort of UA patients. A significant increase of HLA-B15 (p=0.002, OR=18.40) and a moderate increase of HLA-B7 (p=0.043, OR=5.15) was found in patients with UA, but not in patients with ReA. In the B27 negative patients, HLA-DRB104 association with ReA was found independently of B27.

CONCLUSION

Our data confirmed a significant association of HLA-B27 with ReA in the Tunisian population. Our results also suggested that some of the additional HLA antigens were associated with ReA including HLA-B51 and HLA-DRB1*04 alleles. UA seemed to have a genetic background different from ReA in Tunisian patients.

摘要

目的

研究突尼斯反应性关节炎（ReA）和未分化关节炎（UA）患者的HLAⅠ类和Ⅱ类抗原相关性。

方法

该研究纳入了17例根据欧洲脊柱关节病研究组脊柱关节病（SpA）标准定义的ReA患者、11例分类为未分化关节炎的患者以及100名无关健康对照。HLAⅠ类抗原采用血清学方法分型，HLAⅡ类等位基因采用序列特异性引物聚合酶链反应技术进行分子基因分型。

结果

与对照组分别比较时，ReA和UA患者的HLA等位基因存在显著差异。在ReA患者中发现HLA - B27（p = 7.76×10⁻¹²，OR = 59.30）、HLA - B51（p = 0.015，OR = 4.91）和HLA - DRB104（p = 0.033，OR = 2.90）等位基因频率增加，而UA患者中未发现。HLA - B27在我们的UA患者队列中并非完全表达。在UA患者中发现HLA - B15显著增加（p = 0.002，OR = 18.40），HLA - B7中度增加（p = 0.043），而ReA患者中未发现。在B27阴性患者中，发现HLA - DRB104与ReA的关联独立于B27。