突尼斯反应性关节炎和强直性脊柱炎患者中HLA - B27及其等位基因的分布

Distribution of HLA-B27 and its alleles in patients with reactive arthritis and with ankylosing spondylitis in Tunisia.

作者信息

Siala Mariam, Mahfoudh Nadia, Gdoura Radhouane, Younes Mohamed, Fourati Hela, Kammoun Arwa, Chour Ilhem, Meddeb Nihel, Gaddour Lilia, Hakim Faiza, Baklouti Sofien, Bargaoui Naceur, Sellami Sleheddine, Hammami Adnene, Makni Hafedh

机构信息

Laboratoire de Recherche Micro-organismes et Pathologie Humaine, EPS Habib BOURGUIBA de Sfax, Sfax, Tunisia.

出版信息

Rheumatol Int. 2009 Aug;29(10):1193-6. doi: 10.1007/s00296-009-0840-8. Epub 2009 Jan 20.

DOI:10.1007/s00296-009-0840-8

PMID:19152094

Abstract

The purpose of the present study is to investigate the frequency of HLA-B27 and its alleles in reactive arthritis (ReA) and in ankylosing spondylitis (AS) in Tunisia. HLA-B27 alleles were typed by PCR amplification with sequence-specific primers. We studied 17 patients with ReA associated with urethritis or with gastrointestinal infection; 42 HLA-B27-positive patients with AS and 100 healthy controls. Eleven ReA patients (67.7%) were HLA-B27 positive. There was an increased frequencies of HLA-B27 (P = 7.76 x 10(-12), OR = 59.30) and a moderate increase of HLA-B51 (P = 0.015; OR = 4.91) alleles in ReA patients when compared with healthy controls. Four B27 subtypes were identified: B2702, 05, 09 and B2712. The distribution of these alleles in the ReA patients was 37.5% for B2702 and B2705. Only these two subtypes were detected in 18 (42.8%) and 24 (57.1%), respectively, of the AS patients. B2709 and B2712 were relatively rare in ReA patients and were identified in one case each. Our results showed a restricted number of HLA-B27 subtypes associated with ReA and AS. B*2702 and 2705 were common in ReA and AS patients.

摘要

本研究的目的是调查突尼斯反应性关节炎（ReA）和强直性脊柱炎（AS）患者中HLA - B27及其等位基因的频率。采用序列特异性引物PCR扩增法对HLA - B27等位基因进行分型。我们研究了17例与尿道炎或胃肠道感染相关的ReA患者；42例HLA - B27阳性的AS患者以及100名健康对照者。11例ReA患者（67.7%）HLA - B27呈阳性。与健康对照者相比，ReA患者中HLA - B27等位基因频率增加（P = 7.76×10⁻¹²，OR = 59.30），HLA - B51等位基因有中度增加（P = 0.015；OR = 4.91）。鉴定出四种B27亚型：B2702、05、09和B2712。这些等位基因在ReA患者中的分布情况为：B2702占37.5%，B2705占37.5%。在AS患者中，仅这两种亚型分别在18例（42.8%）和24例（57.1%）中检测到。B2709和B2712在ReA患者中相对少见，各仅在1例中鉴定出。我们的结果显示，与ReA和AS相关的HLA - B27亚型数量有限。B*2702和2705在ReA和AS患者中较为常见。