FN13762小鼠乳腺癌：首过灌注CT指标与组织学血管参数的逐区域相关性

FN13762 murine breast cancer: region-by-region correlation of first-pass perfusion CT indexes with histologic vascular parameters.

作者信息

Park Chang Min, Goo Jin Mo, Lee Hyun Ju, Kim Min A, Kim Hyo-Cheol, Kim Kwang Gi, Lee Chang Hyun, Im Jung-Gi

机构信息

Department of Radiology, Seoul National University College of Medicine, and Institute of Radiation Medicine, Seoul National University Medical Research Center, 101 Daehangno, Jongno-gu, Seoul 110-744, Korea.

出版信息

Radiology. 2009 Jun;251(3):721-30. doi: 10.1148/radiol.2513081215.

DOI:10.1148/radiol.2513081215

PMID:19474375

Abstract

PURPOSE

To investigate the correlation between first-pass perfusion computed tomographic (CT) indexes and histologic vascular parameters in FN13762 breast cancer in rats by using region-by-region correlation methods.

MATERIALS AND METHODS

The Animal Care and Use Committee approved this study. FN13762 murine breast cancer cells were implanted in 14 female Fischer 344 rats, and first-pass perfusion CT was performed. CT perfusion maps depicting blood flow, blood volume, mean transit time (MTT), and permeability-surface area (PSA) product were generated by using commercial perfusion software. The entire tumor area was divided into six separate regions on perfusion maps, and the regional perfusion indexes were quantified. Histologic vascular parameters, including microvessel density (MVD), luminal vessel number, luminal vessel area, and luminal vessel perimeter, were measured in the histologic region corresponding to the perfusion maps. Correlation analysis was performed between regional tumor perfusion indexes and histologic vascular parameters of the corresponding tumor region. Additionally, mean perfusion values of the entire tumor were correlated with histologic vascular parameters of the hot spot within the tumor. Among 14 rats, four were excluded from the analysis, and results were based on a final total of 10 rats.

RESULTS

In tumors, blood flow, blood volume, and PSA product were significantly higher and MTT was significantly shorter (P < .05 for all) than these values in normal neck muscles. At region-by-region correlation, regional blood flow (r = 0.476), blood volume (r = 0.348), and MTT (r = -0.506) were significantly correlated with MVD in the corresponding tumor region (P < .01 for all). After adjustment for biologic variability between rats, regional blood flow (r = 0.614), blood volume (r = 0.515), MTT (r = -0.524), and PSA product (r = 0.228) remained significantly correlated with MVD in the corresponding tumor region. Correlation analysis between CT perfusion indexes of the entire tumor and histologic vascular parameters of the hot spot did not show significant correlations (P > .05).

CONCLUSION

Regional blood flow, blood volume, and MTT are significantly correlated with MVD in the corresponding tumor region.

摘要

目的

采用逐区域相关分析方法，研究大鼠FN13762乳腺癌首次通过灌注计算机断层扫描（CT）指标与组织学血管参数之间的相关性。

材料与方法

本研究经动物护理与使用委员会批准。将FN13762小鼠乳腺癌细胞接种于14只雌性Fischer 344大鼠体内，并进行首次通过灌注CT检查。使用商用灌注软件生成描绘血流、血容量、平均通过时间（MTT）和通透表面积（PSA）乘积的CT灌注图。在灌注图上将整个肿瘤区域划分为六个独立区域，并对区域灌注指标进行量化。在与灌注图相对应的组织学区域测量组织学血管参数，包括微血管密度（MVD）、管腔血管数量、管腔血管面积和管腔血管周长。对区域肿瘤灌注指标与相应肿瘤区域的组织学血管参数进行相关分析。此外，将整个肿瘤的平均灌注值与肿瘤内热点区域的组织学血管参数进行相关性分析。14只大鼠中，4只被排除在分析之外，最终结果基于总共10只大鼠。

结果

在肿瘤中，血流、血容量和PSA乘积显著高于正常颈部肌肉，MTT显著短于正常颈部肌肉（所有P < 0.05）。在逐区域相关分析中，区域血流（r = 0.476）、血容量（r = 约0.348）和MTT（r = -0.506）与相应肿瘤区域的MVD显著相关（所有P < 0.01）。在调整大鼠之间的生物学变异性后，区域血流（r = 0.614）、血容量（r = 0.515）、MTT（r = -0.524）和PSA乘积（r = 0.228）仍与相应肿瘤区域的MVD显著相关。整个肿瘤的CT灌注指标与热点区域的组织学血管参数之间的相关分析未显示出显著相关性（P > 0.05）。