Matsunaga Tadashi, Matsumoto Mitsufumi, Maeda Yoshiaki, Sugiyama Hiroshi, Sato Reiko, Tanaka Tsuyoshi
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
Biotechnol Lett. 2009 Sep;31(9):1367-72. doi: 10.1007/s10529-009-0029-y. Epub 2009 May 28.
A marine microalga, strain JPCC GA0024 was selected as high amount of neutral lipid producers from marine microalgal culture collection toward biofuel production. The strain was tentatively identified as Scenedesmus rubescens by 18S rDNA analysis. The growth of strain JPCC GA0024 was influenced by artificial seawater concentrations. The optimum growth of 0.79 g/l was obtained at 100% artificial seawater. The lipid accumulation reached 73.0% of dry cell weight at 100% artificial seawater without additional nutrients for 11 days. Gas chromatography/mass spectrometry analysis indicates that lipid fraction mainly contained hydrocarbons including mainly hexadecane (C(16) H(34)) and 1-docosene (C(22) H(44)). Furthermore, calorimetric analysis revealed that the energy content of strain JPCC GA0024 was 6,160 kcal/kg (25.8 MJ/kg) of calorific value, which was equivalent to the coal engery. The strain JPCC GA0024, S. rubescens, will become a promising resource that can grow as a dominant species in the seawater for the production of both liquid and solid biofuels.
从海洋微藻培养物中筛选出一株海洋微藻JPCC GA0024，作为用于生物燃料生产的高产中性脂质的微藻。通过18S rDNA分析，该菌株初步鉴定为红栅藻。JPCC GA0024菌株的生长受人工海水浓度的影响。在100%人工海水中获得的最佳生长量为0.79 g/l。在100%人工海水中，不添加额外营养物质培养11天，脂质积累量达到干细胞重量的73.0%。气相色谱/质谱分析表明，脂质部分主要包含碳氢化合物，主要为十六烷（C(16) H(34)）和1-二十二碳烯（C(22) H(44)）。此外，量热分析显示，JPCC GA0024菌株的能量含量为6160千卡/千克（25.8兆焦/千克），相当于煤炭能量。JPCC GA0024菌株，即红栅藻，将成为一种有前景的资源，它可以在海水中作为优势物种生长，用于生产液体和固体生物燃料。
