Fernius Josefin, Hardwick Kevin G
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol. 2009;545:243-58. doi: 10.1007/978-1-60327-993-2_15.
The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation by inhibiting anaphase onset until all chromosomes have established stable bipolar attachments. Here we describe a number of protocols that can be used to assay the ability of budding and fission yeast cells to (1) establish and maintain a spindle checkpoint arrest, and (2) segregate chromosomes efficiently upon recovery from mitotic arrest. We focus on experimental detail of the budding yeast protocols, but also point out important differences between budding and fission yeast assays.
纺锤体检查点是一种监测机制，通过抑制后期开始来确保染色体分离的准确性，直到所有染色体都建立稳定的双极附着。在这里，我们描述了一些可用于检测出芽酵母细胞和裂殖酵母细胞的能力的实验方案，这些能力包括：（1）建立并维持纺锤体检查点阻滞，以及（2）从有丝分裂阻滞中恢复后有效分离染色体。我们重点关注出芽酵母实验方案的实验细节，但也指出了出芽酵母和裂殖酵母检测之间的重要差异。
