Liu Yang, Li Buhai, Sun Xiaomei, Lin Aihua
School of Life Science and Technology, Wuhan University of Science and Technology Zhongnan Branch, 430223 Wuhan, Hubei, People's Republic of China.
Protein J. 2009 May;28(3-4):169-74. doi: 10.1007/s10930-009-9180-5.
Cardiac myosin is the most abundant and bioactive protein presented in heart tissue. In this work, a simple method was described for preparation of high purity cardiac myosin for further research. With the use of a domestic pig left ventricle as the starting tissue, this method yielded 4.5 mg myosin per gram of wet tissue with a specific activity of 0.293 U/mg. The purification factor was 4.9-fold with an activity recovery of 42.1%. In addition, the investigation of fluorescence spectrum shown that the emission intensity of cardiac myosin has a dramatic decrease under the influence of an acidic environment or at temperatures higher than 30 degrees C, which could influence the normal physiological function of cardiac myosin.
