Brunelli M, Gobbo S, Cossu-Rocca P, Cheng L, Ficarra V, Novara G, Menestrina F, Chilosi M, Martignoni G
Department of Pathology, University of Verona, Italy.
Pathologica. 2008 Dec;100(6):454-60.
Renal cell neoplasms are a heterogeneous group of tumours in terms of pathological features and prognostic behaviour. The genetics of these tumours may aid in correct diagnosis and accurate assessment of prognosis. In ambiguous cases it may be necessary to utilise new markers that are capable of further discerning renal cell neoplasms. Fluorescence in situ hybridization (FISH) on formalin-fixed, paraffin-embedded tissue is an increasingly useful technique in the detection of many diagnostic chromosomal abnormalities, among which chromosomes 1, 2, 3p, 6, 7, 10, 17 and Y are the most common. The addition of FISH to histological evaluation improves the diagnostic accuracy of core biopsies from renal masses, which may have an important impact in clinical management of many cases due to newer therapeutic approaches, including cryo- or radiofrequency ablation, nephron-sparing surgeries and target therapies.
肾细胞肿瘤在病理特征和预后表现方面是一组异质性肿瘤。这些肿瘤的遗传学特征有助于正确诊断和准确评估预后。在诊断不明确的病例中，可能有必要使用能够进一步区分肾细胞肿瘤的新标志物。对福尔马林固定、石蜡包埋组织进行荧光原位杂交（FISH）是检测许多诊断性染色体异常越来越有用的技术，其中1、2、3p、6、7、10、17号染色体和Y染色体最为常见。将FISH添加到组织学评估中可提高肾肿块核心活检的诊断准确性，由于包括冷冻或射频消融、保留肾单位手术和靶向治疗在内的新治疗方法，这可能对许多病例的临床管理产生重要影响。
