Goodwin Erin M, Zhong Qing, Abendroth Catherine S, Ward-Kavanagh Lindsay K, Schell Todd D, Cooper Timothy K
Department of Comparative Medicine, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Comp Med. 2013 Aug;63(4):338-41.
An 8-mo-old female transgenic adenocarcinoma of the mouse prostate (C57BL/6-Tg(TRAMP)8247Ng/J) mouse presented with abdominal distention, lethargy, and serosanguineous vaginal discharge. A large primary renal tumor with metastases to lung and liver was present at necropsy. The tumor was composed of poorly differentiated and crowded epithelial cells forming ducts, acini, and cribriform patterns, with comedonecrosis and frequent bizarre mitoses. Immunohistochemistry revealed that neoplastic cells expressed nuclear SV40 T antigen, confirming aberrant expression of the transgene. In addition, cells were positive for pancytokeratin and negative for synaptophysin and estrogen and progesterone receptors. This report details the first transgene-induced tumor in a female TRAMP mouse.
一只8月龄的雌性转基因小鼠前列腺腺癌（C57BL/6-Tg(TRAMP)8247Ng/J）出现腹胀、嗜睡和血性阴道分泌物。尸检发现一个大的原发性肾肿瘤并伴有肺和肝转移。肿瘤由分化差且密集的上皮细胞组成，形成导管、腺泡和筛状结构，伴有粉刺样坏死和频繁的怪异有丝分裂。免疫组织化学显示肿瘤细胞表达核SV40 T抗原，证实转基因异常表达。此外，细胞全细胞角蛋白呈阳性，突触素、雌激素和孕激素受体呈阴性。本报告详细描述了首例雌性TRAMP小鼠中由转基因诱导的肿瘤。
