Lacikova Lubica, Pferschy-Wenzig Eva-Maria, Masterova Irena, Grancai Daniel, Bauer Rudolf
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, Slovakia.
Nat Prod Commun. 2009 Apr;4(4):543-6.
Staphylea preparations are used in TCM and have been used by native Americans for a number of indications, such as rheumatism. Based on this knowledge, the anti-inflammatory activity of light petroleum extracts of leaves of Staphylea colchica Stev., S. elegans Zab., S. holocarpa Hemsl. and S. pinnata L. has been determined using in vitro assays for inhibition of cyclooxygenase (COX-1 and COX-2) and leukotriene B4 (LTB4) formation by lipoxygenase (5-LOX). All extracts inhibited COX-1 and COX-2, with S. holocarpa and S. elegans performing best. Inhibition of LTB4 formation was less pronounced. As unsaturated fatty acids are known to inhibit arachidonic acid metabolism in vitro, the fatty acid content was determined of the active extracts and set in correlation with their activity. Unsaturated fatty acids were found to contribute to the observed COX-2 and LTB4 formation inhibitory activity to a different extent.
省沽油制剂在传统中医中使用，美洲原住民也将其用于多种适应症，如治疗风湿病。基于这一认知，运用体外试验测定了光叶省沽油、秀丽省沽油、全果省沽油和羽叶省沽油叶片的石油醚提取物对环氧化酶（COX-1和COX-2）的抑制作用以及对脂氧合酶（5-LOX）生成白三烯B4（LTB4）的抑制作用，以此来确定其抗炎活性。所有提取物均对COX-1和COX-2有抑制作用，其中全果省沽油和秀丽省沽油的效果最佳。对LTB4生成的抑制作用则不太显著。由于已知不饱和脂肪酸在体外可抑制花生四烯酸代谢，因此测定了活性提取物中的脂肪酸含量，并将其与活性进行关联。结果发现，不饱和脂肪酸在不同程度上对所观察到的COX-2和LTB4生成抑制活性有贡献。
