Bryan Laura K, Baldwin Charles A, Gray Matthew J, Miller Debra L
University of Georgia, College of Veterinary Medicine, Athens, Georgia 30602, USA.
Dis Aquat Organ. 2009 Apr 6;84(2):89-94. doi: 10.3354/dao02036.
Ranavirus can cause disease in reptiles and amphibians. Because survival time outside of a host remains uncertain, equipment must be disinfected to prevent transmission of ranaviruses. However, disinfectant efficacy against amphibian ranaviruses has not been investigated for chlorhexidine (Nolvasan), sodium hypochlorite (bleach), or potassium compounds. Our goal was to determine the efficacy of Nolvasan (0.25, 0.75 and 2.0%), bleach (0.2, 1.0, 3.0 and 5.0%), and Virkon S (1.0%) at inactivating Ranavirus at 1 and 5 min contact durations. Potassium permanganate (KMnO4) (2.0 and 5.0 ppm) was also tested with a 60 min contact time. Nolvasan at 0.75 and 2.0% and bleach at 3.0 and 5.0% concentration were effective for both contact durations. Virkon S was effective for both durations, but KMnO4 was not effective at either concentration. Concentrations of Nolvasan, bleach and Virkon S that are at least 0.75, 3.0 and 1.0%, respectively, are effective at inactivating Ranavirus after 1 min exposure time.
蛙病毒可导致爬行动物和两栖动物患病。由于在宿主体外的存活时间尚不确定，设备必须进行消毒以防止蛙病毒传播。然而，尚未对洗必泰（诺伏沙星）、次氯酸钠（漂白剂）或钾化合物针对两栖动物蛙病毒的消毒效果进行研究。我们的目标是确定诺伏沙星（0.25%、0.75%和2.0%）、漂白剂（0.2%、1.0%、3.0%和5.0%）以及卫可Virkon S（1.0%）在接触1分钟和5分钟时灭活蛙病毒的效果。高锰酸钾（KMnO₄）（2.0 ppm和5.0 ppm）也在接触60分钟时进行了测试。0.75%和2.0%的诺伏沙星以及3.0%和5.0%的漂白剂在两种接触时长下均有效。卫可Virkon S在两种时长下均有效，但高锰酸钾在两种浓度下均无效。诺伏沙星、漂白剂和卫可Virkon S的浓度分别至少为0.75%、3.0%和1.0%时，在接触1分钟后对灭活蛙病毒有效。
