Bhang Suk Ho, Won Nayoun, Lee Tae-Jin, Jin Ho, Nam Jutaek, Park Joonhyuck, Chung Hyokyun, Park Hyun-Seo, Sung Yung-Eun, Hahn Sei Kwang, Kim Byung-Soo, Kim Sungjee
Department of Bioengineering, Hanyang University, Seoul 133-791, Korea.
ACS Nano. 2009 Jun 23;3(6):1389-98. doi: 10.1021/nn900138d.
A simple and novel electrostatic coupling method is reported, which provides a hyaluronic acid-quantum dot conjugate (HA-QD) that is colloidally stable and size-tunable from 50 to 120 nm. The HA-QDs show cancer targeting efficiency, which suggests diagnostic and imaging applications. The conjugates are also demonstrated for the fluorescence staining capability for lymphatic vessels in vitro and in vivo. Using the HA-QDs in a small animal model, lymphatic vessels are visualized real-time in vivo for days. Comprehensive cytotoxicity evaluations are made for the conjugates and the unconjugated counterpart. The HA-QDs showcase the potentials toward cancer imaging and real-time visualization of changes in lymphatic vessels such as lymphangiogenesis.
报道了一种简单且新颖的静电偶联方法，该方法可提供一种透明质酸-量子点共轭物（HA-QD），其具有胶体稳定性，粒径可在50至120纳米之间调节。HA-QD显示出癌症靶向效率，这表明其具有诊断和成像应用潜力。这些共轭物还在体外和体内展示了对淋巴管的荧光染色能力。在小动物模型中使用HA-QD，可在体内实时观察淋巴管达数天之久。对共轭物和未偶联的对应物进行了全面的细胞毒性评估。HA-QD展现出在癌症成像以及实时可视化淋巴管变化（如淋巴管生成）方面的潜力。
