Cheng Hsiu-Ting, Chuang Er-Yuan, Ma Hsu, Tsai Chi-Han, Perng Cherng-Kang
Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taiwan, Republic of China.
Ann Plast Surg. 2012 Dec;69(6):663-7. doi: 10.1097/SAP.0b013e3182746787.
A quantum dot (QD)-conjugated collagen-hyaluronic acid (HA) porous scaffold was combined with our previously reported animal model of mice inferior epigastric flaps and QD infusion to study scaffold angiogenesis. A CdSe/ZnS QD-labeled collagen/HA scaffold was fabricated and examined with a confocal microscope. The degradation rate of the scaffold was inversely related to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide concentration used in cross-linking. There was no cytotoxicity of the QDs as determined by MTT colorimetric assay. Results of the animal implantation study revealed no difference in acute tissue inflammation between scaffolds with or without QD labeling. To study scaffold angiogenesis, we infused the implanted QD-conjugated collagen/HA scaffold with QD of different fluorescence, which can be simultaneously identified by confocal microscope. By these evaluations, we conclude that QD-conjugated collagen/HA porous scaffold is not different from that without conjugation and can be used in our animal model of scaffold angiogenesis without compromising results.
将量子点（QD）共轭的胶原蛋白-透明质酸（HA）多孔支架与我们之前报道的小鼠腹壁下皮瓣动物模型及量子点注入相结合，以研究支架血管生成。制备了CdSe/ZnS量子点标记的胶原蛋白/HA支架并用共聚焦显微镜进行检测。支架的降解速率与交联中使用的1-乙基-3-（3-二甲基氨基丙基）碳二亚胺浓度呈负相关。通过MTT比色法测定，量子点没有细胞毒性。动物植入研究结果显示，有或没有量子点标记的支架之间在急性组织炎症方面没有差异。为了研究支架血管生成，我们向植入的量子点共轭胶原蛋白/HA支架中注入不同荧光的量子点，这些量子点可通过共聚焦显微镜同时识别。通过这些评估，我们得出结论，量子点共轭胶原蛋白/HA多孔支架与未共轭的支架没有差异，可用于我们的支架血管生成动物模型，而不会影响结果。
