Kabuto M, Suzuki K, Ohsawa N, Kosaka K
Endocrinol Jpn. 1977 Apr;24(2):173-8. doi: 10.1507/endocrj1954.24.173.
A simple radioreceptor assay for insulin rat liver membranes as receptor sites, with sufficient specificity precision, and sensitivity to detect 10 ng or 276 muU/ml of serum insulin, has been developed. In the presence of standard porcine insulin at the concentration of 1.0 ng/tube, approximately 8% of 125I-porcine insulin was bound to the plasma membranes and ninety-five per cent of this binding was inhibited by 1.0 microgram of standard insulin per tube. Four animal insulins inhibited the binding of 125I-insulin while ACTH, glucagon, human growth hormone, and oxytocin were inert. Insulin values in dog pancreatic vein sera obtained during and after glucose loading and measured by the present radioreceptor assay agreed well with immunoreactive insulin. The ratio of IRI to the measurement by radioreceptor assay was 1.09 +/- 0.18 for the same sera.
已开发出一种简单的放射受体测定法，以大鼠肝细胞膜作为胰岛素的受体位点，该方法具有足够的特异性、精密度和灵敏度，可检测10 ng或276 μU/ml的血清胰岛素。在每管含有1.0 ng标准猪胰岛素的情况下，约8%的125I - 猪胰岛素与质膜结合，每管1.0 μg标准胰岛素可抑制95%的这种结合。四种动物胰岛素可抑制125I - 胰岛素的结合，而促肾上腺皮质激素、胰高血糖素、人生长激素和催产素则无活性。通过本放射受体测定法测得的葡萄糖负荷期间及之后犬胰静脉血清中的胰岛素值与免疫反应性胰岛素值吻合良好。同一血清的免疫反应性胰岛素（IRI）与放射受体测定法测量值的比值为1.09±0.18。
