Gavin J R, Kahn C R, Gorden P, Roth J, Neville D M
J Clin Endocrinol Metab. 1975 Sep;41(3):438-45. doi: 10.1210/jcem-41-3-438.
Porcine proinsulin, related intermediates and plasma immunoreactive insulin components have been studied by radioreceptor assay. Using the purified rat liver membrane or cultured human lymphocyte radioreceptor assay, porcine proinsulin is %5, split proinsulin 6% (54-55 split in connecting peptide) desdipeptide proinsulin 20% (deletion of amino acids 62 and 63 of connecting peptide) and desnonapeptide proinsulin 27% (deletion of amino acids 55-63 of connecting peptide) as active as porcine insulin in both assay systems; these values closely parallel the in vitro bioactivity of these preparations. In the lymphocyte radioreceptor assay the human plasma immunoreactive insulin-like component has the same potency as porcine insulin per immunoreactive unit, whereas the plasma immunoreactive proinsulin-like component is only 15% as active. Since both plasma immunoreactive components are somewhat less reactive than would be expected from puriified human insulin and proinsulin, the data suggest that both plasma components contain immunoreactive molecules that do not react in the radioreceptor assay.
已通过放射受体分析法对猪胰岛素原、相关中间体及血浆免疫反应性胰岛素成分进行了研究。使用纯化的大鼠肝细胞膜或培养的人淋巴细胞放射受体分析法，在两种分析系统中，猪胰岛素原的活性为猪胰岛素的5%，裂解胰岛素原的活性为6%（连接肽中54 - 55位裂解），去二肽胰岛素原的活性为20%（连接肽中氨基酸62和63缺失），去九肽胰岛素原的活性为27%（连接肽中氨基酸55 - 63缺失）；这些数值与这些制剂的体外生物活性密切平行。在淋巴细胞放射受体分析法中，人血浆免疫反应性胰岛素样成分每免疫反应单位的效力与猪胰岛素相同，而血浆免疫反应性胰岛素原样成分的活性仅为15%。由于两种血浆免疫反应性成分的反应性均略低于纯化的人胰岛素和胰岛素原所预期的反应性，数据表明两种血浆成分均含有在放射受体分析法中不发生反应的免疫反应性分子。
