Ginther O J, Siddiqui M A R, Beg M A
Eutheria Foundation, Cross Plains, WI 53528, USA; Pathobiological Sciences, University of Wisconsin, Madison, WI 53706, USA.
Theriogenology. 2009 Aug;72(3):417-24. doi: 10.1016/j.theriogenology.2009.03.014. Epub 2009 May 23.
Responses to intravenous treatment of mares with prostaglandin F2alpha (PGF) 8 d after ovulation were studied in three groups (n=4/group): control (no treatment), bolus (single treatment with 2.5 mg PGF), and infusion (0.1 mg PGF during 2 h). Infusion resulted in a 13,14-dihydro-15-keto-PGF2alpha (PGFM) concentration (559+/-44 pg/mL) that was not different from the mean concentration for the major portion of a natural PGFM pulse associated with luteolysis (569+/-45 pg/mL; n=5). Progesterone in the bolus group increased (P<0.03) between 0 (17.8+/-3.5 ng/mL) and 2 min (25.3+/-4.8 ng/mL), peaked at 10 min (28.5+/-4.6 ng/mL), and then decreased. In the infusion group, progesterone decreased (P<0.05) during 1 min (17.2+/-1.3 ng/mL) to 15 min (13.5+/-1.5 ng/mL) after the beginning of infusion and decreased (P<0.05) similarly to the bolus group during 2 to 12 h; concentrations were lower (P<0.05) at each hour than in controls. Interval between ovulations was shorter (P<0.05) in the bolus (19.3+/-2.0 d) and infusion (18.8+/-2.1 d) groups than in controls (24.3+/-1.3 d). Concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and cortisol increased (P<0.05) within 10 min in the bolus group but did not change in the infusion group. Results supported the hypothesis that increases in progesterone, FSH, LH, and cortisol after a bolus luteolytic PGF treatment are nonphysiologic. Past conclusions on the nature of the luteolytic mechanism are problematic if based on responses to treatment with a single luteolytic bolus of PGF.
在排卵后8天，对三组（每组n = 4）母马进行前列腺素F2α（PGF）静脉注射治疗，并观察其反应：对照组（不治疗）、推注组（单次注射2.5 mg PGF）和输注组（2小时内输注0.1 mg PGF）。输注导致13,14 - 二氢 - 15 - 酮 - PGF2α（PGFM）浓度（559±44 pg/mL），与与黄体溶解相关的自然PGFM脉冲主要部分的平均浓度（569±45 pg/mL；n = 5）无差异。推注组中孕酮在0（17.8±3.5 ng/mL）至2分钟（25.3±4.8 ng/mL）之间升高（P<0.03），在10分钟时达到峰值（28.5±4.6 ng/mL），然后下降。在输注组中，输注开始后1分钟（17.2±1.3 ng/mL）至15分钟（13.5±1.5 ng/mL）期间孕酮下降（P<0.05），并且在2至12小时期间与推注组类似下降（P<0.05）；每小时的浓度均低于对照组（P<0.05）。推注组（19.3±2.0天）和输注组（18.8±2.1天）的排卵间隔比对照组（24.3±1.3天）短（P<0.05）。推注组中促卵泡激素（FSH）、促黄体生成素（LH）和皮质醇的浓度在10分钟内升高（P<0.05），但输注组中未发生变化。结果支持以下假设：推注黄体溶解PGF治疗后孕酮、FSH、LH和皮质醇的升高是非生理性的。如果基于对单次推注黄体溶解PGF治疗的反应得出关于黄体溶解机制性质的过去结论，则存在问题。
