Sun Fuhong, Zhou Qixing, Wang Meie, An Jing
Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, People's Republic of China.
Ecotoxicol Environ Saf. 2009 Oct;72(7):1887-92. doi: 10.1016/j.ecoenv.2009.04.017. Epub 2009 May 23.
Responses of the polychaete Perinereis aibuhitensis Grube to single and joint stress of copper (Cu) and petroleum hydrocarbons (PHCs) at biochemical levels (including superoxide dismutases, SOD; peroxidases, POD; and the content of total soluble protein, TSP) were investigated under controlled laboratorial conditions. Significant decreases in SOD activity in the first 3 days of single exposure to Cu or PHCs were observed. Although SOD activity increased and returned to the level of the control on day 6 after having decreased on day 3 under single stress of Cu or PHCs, Cu and PHCs had complicated interactive influences on SOD activity in P. aibuhitensis. Their joint effects on SOD activity depended on concentration combinations and exposure time, called double-dose dependent effects. The POD activity and the content of TSP remained generally constant under both single and joint stress of Cu and/or PHCs, compared with the control. The beef-witted responses of investigated biochemical parameters in this study might suggest that toxic effects of pollutants with emphasis on the action of abiotic parameters and the time factor on the antioxidant enzymes in the polychaete should be addressed in the future work.
在实验室控制条件下，研究了多毛纲动物艾氏围沙蚕（Perinereis aibuhitensis Grube）对铜（Cu）和石油烃（PHCs）单一及联合胁迫在生化水平（包括超氧化物歧化酶，SOD；过氧化物酶，POD；以及总可溶性蛋白含量，TSP）上的响应。观察到在单一暴露于Cu或PHCs的前3天，SOD活性显著下降。尽管在Cu或PHCs单一胁迫下，SOD活性在第3天下降后于第6天增加并恢复到对照水平，但Cu和PHCs对艾氏围沙蚕的SOD活性具有复杂的交互影响。它们对SOD活性的联合效应取决于浓度组合和暴露时间，称为双剂量依赖效应。与对照相比，在Cu和/或PHCs的单一及联合胁迫下，POD活性和TSP含量总体保持恒定。本研究中所研究生化参数的迟钝响应可能表明，在未来的工作中应关注污染物的毒性效应，重点是 abiotic参数的作用以及时间因素对多毛纲动物抗氧化酶的影响。
