Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China.
Mar Pollut Bull. 2012 Sep;64(9):1782-8. doi: 10.1016/j.marpolbul.2012.05.035. Epub 2012 Jul 5.
A CYP4 cDNA was cloned and characterized to identify the relationship between persistent organic pollutants and stress response in marine polychaete Perinereis aibuhitensis. The full length of PaCYP4 cDNA is 1857bp and encodes 481 amino acids. The deduced amino acid sequence showed 73% identity with CYP4BB1 from polychaete Nereis virens and shared high homology to other known CYP4 sequences. The expression level of PaCYP4 under petroleum hydrocarbon (PH) and deltamethrin (DM) exposure was detected using Real-time PCR. PH and combined toxicity treatments elevated the mRNA level of PaCYP4 in a dose- and time-dependent manner. The mRNA transcripts of PaCYP4 increased at the beginning of DM exposure and then eventually decreased, and the expression level of PaCYP4 down-regulated with increasing concentration of DM. CYP4 in P. aibuhitensis plays an important role in the metabolism of petroleum hydrocarbon and organochlorine pesticide.
克隆并鉴定了一个 CYP4 cDNA，以研究海洋多毛类环节动物沙蚕（Perinereis aibuhitensis）中持久性有机污染物与应激反应之间的关系。PaCYP4 cDNA 的全长为 1857bp，编码 481 个氨基酸。推导的氨基酸序列与多毛类环节动物沙蚕（Nereis virens）的 CYP4BB1 具有 73%的同一性，并与其他已知的 CYP4 序列具有高度同源性。采用 Real-time PCR 检测了 PaCYP4 在石油烃（PH）和溴氰菊酯（DM）暴露下的表达水平。PH 和联合毒性处理以剂量和时间依赖的方式升高 PaCYP4 的 mRNA 水平。DM 暴露初期 PaCYP4 的 mRNA 转录本增加，随后逐渐减少，随着 DM 浓度的增加，PaCYP4 的表达水平下调。沙蚕中的 CYP4 在石油烃和有机氯农药的代谢中发挥重要作用。
