黏液瘤病毒M130R是兔致命性黏液瘤病所需的一种新型毒力因子。

Myxoma virus M130R is a novel virulence factor required for lethal myxomatosis in rabbits.

作者信息

Barrett John W, Werden Steven J, Wang Fuan, McKillop William M, Jimenez June, Villeneuve Danielle, McFadden Grant, Dekaban Gregory A

机构信息

Biotherapeutics Research Group, Robarts Research Institute and Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5K8, Canada.

出版信息

Virus Res. 2009 Sep;144(1-2):258-65. doi: 10.1016/j.virusres.2009.05.009. Epub 2009 May 27.

DOI:10.1016/j.virusres.2009.05.009

PMID:19477207

Abstract

Myxoma virus (MV) is a highly lethal, rabbit-specific poxvirus that induces a disease called myxomatosis in European rabbits. In an effort to understand the function of predicted immunomodulatory genes we have deleted various viral genes from MV and tested the ability of these knockout viruses to induce lethal myxomatosis. MV encodes a unique 15 kD cytoplasmic protein (M130R) that is expressed late (12h post infection) during infection. M130R is a non-essential gene for MV replication in rabbit, monkey or human cell lines. Construction of a targeted gene knockout virus (vMyx130KO) and infection of susceptible rabbits demonstrate that the M130R knockout virus is attenuated and that loss of M130R expression allows the rabbit host immune system to effectively respond to and control the lethal effects of MV. M130R expression is a bona fide poxviral virulence factor necessary for full and lethal development of myxomatosis.

摘要

黏液瘤病毒（MV）是一种高度致命的、专性感染兔子的痘病毒，可在欧洲兔中引发一种名为黏液瘤病的疾病。为了了解预测的免疫调节基因的功能，我们从MV中删除了各种病毒基因，并测试了这些基因敲除病毒诱导致命性黏液瘤病的能力。MV编码一种独特的15kD细胞质蛋白（M130R），该蛋白在感染后期（感染后12小时）表达。M130R对于MV在兔、猴或人细胞系中的复制而言并非必需基因。构建靶向基因敲除病毒（vMyx130KO）并感染易感兔，结果表明M130R基因敲除病毒的毒力减弱，且M130R表达缺失使兔宿主免疫系统能够有效应对并控制MV的致死效应。M130R表达是黏液瘤病充分发展及致死所必需的一种真正的痘病毒毒力因子。