Vu Thi Huong, Shimanouchi Toshinori, Ishii Haruyuki, Umakoshi Hiroshi, Kuboi Ryoichi
Division of Chemical Engineering, Department of Engineering Science, Graduate School of Engineering Science, Osaka University, Japan.
J Colloid Interface Sci. 2009 Aug 15;336(2):902-7. doi: 10.1016/j.jcis.2009.04.048. Epub 2009 Apr 23.
Immobilization of model cell membranes (liposomes) on sensing devices is useful for the detection, evaluation, and analysis of interaction between cell membranes and proteins or other biological materials. The method to immobilize the liposomes on the solid surface was investigated by using the quartz crystal microbalance (QCM) method, focusing on the density of immobilized liposomes and their stability. The different liposomes were applied onto the planar surface of the QCM electrode by varying the immobilization method. The results showed that the immobilized liposomes depended not only on the immobilization method but also on the properties of the liposomes. The liposome with low membrane fluidity likely showed intact immobilization on a solid surface. An electrostatic interaction also affected the amount of immobilized liposomes on the functionalized quartz crystal. By using the amino-coupling method, intact liposomes could be immobilized on a solid surface and immobilized liposomes could stabilize for 10 h with frequency changes less than 5%.
将模型细胞膜（脂质体）固定在传感装置上，有助于检测、评估和分析细胞膜与蛋白质或其他生物材料之间的相互作用。利用石英晶体微天平（QCM）方法研究了将脂质体固定在固体表面的方法，重点关注固定化脂质体的密度及其稳定性。通过改变固定方法，将不同的脂质体应用于QCM电极的平面表面。结果表明，固定化脂质体不仅取决于固定方法，还取决于脂质体的性质。膜流动性低的脂质体可能在固体表面完整固定。静电相互作用也影响功能化石英晶体上固定化脂质体的数量。通过氨基偶联法，完整的脂质体可以固定在固体表面，固定化脂质体可以稳定10小时，频率变化小于5%。
