感染虹彩病毒的条石鲷（Oplegnathus fasciatus；Temminck和Schlegel）中病毒分布的特征分析

Characterization of virus distribution in Rock Bream (Oplegnathus fasciatus; Temminck and Schlegel) infected with megalocytivirus.

作者信息

Lee N-S, Do J W, Park J W, Kim Y C

机构信息

Pathology Division, National Fisheries Research & Development Institute, Busan, South Korea.

出版信息

J Comp Pathol. 2009 Jul;141(1):63-9. doi: 10.1016/j.jcpa.2009.03.008. Epub 2009 May 28.

DOI:10.1016/j.jcpa.2009.03.008

PMID:19477463

Abstract

The distribution of virus-infected cells in the organs of Rock Bream naturally infected with megalocytivirus is reported. Examination of sections of liver, spleen and kidney stained by haematoxylin and eosin (HE) and periodic acid Schiff (PAS) revealed the presence of swollen and degenerate cells having morphology consistent with leucocytes. Many of these cells were shown to contain viral DNA by in-situ hybridization (ISH). Cells containing viral DNA were also found in the connective tissue of other organs in which there was no prominent infiltrate of degenerate leucocytes. Viral DNA was also found in the cytoplasm of leucocytes in blood smears. Transmission electron microscopy (TEM) confirmed the presence of viral particles in the cells within tissue and free within blood. The tissue distribution of virus in this infection is suggested to reflect the infiltration of virus-infected leucocytes.

摘要

报道了自然感染巨细胞病毒的条石鲷器官中病毒感染细胞的分布情况。对经苏木精和伊红（HE）染色以及过碘酸希夫（PAS）染色的肝脏、脾脏和肾脏切片进行检查，发现存在形态与白细胞一致的肿胀和退化细胞。通过原位杂交（ISH）显示，其中许多细胞含有病毒DNA。在其他器官的结缔组织中也发现了含有病毒DNA的细胞，这些器官中没有明显的退化白细胞浸润。在血液涂片的白细胞细胞质中也发现了病毒DNA。透射电子显微镜（TEM）证实组织内细胞和血液中游离的细胞内存在病毒颗粒。这种感染中病毒的组织分布表明反映了病毒感染白细胞的浸润情况。